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product information
|
| background |
|
RA - Ribulose bisphosphate carboxylase/oxygenase activase is an enzyme localized to chloroplasts which activates Rubisco by promoting ATP-dependent conformational changes. Alternative name: RuBisCo activase RCA. |
| immunogen |
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purified, recombinant Rubisco activase from Gossypium hirsutum Q9AXG1 |
| antibody format |
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rabbit |
polyclonal |
|
serum |
lyophilized |
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| quantity |
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100 µl |
for reconstitution add 100 µl, of sterile water. |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
antibodies to RbcL, RbcS |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1 : 5000 - 1: 10 000 with NBT/BCIP (WB) |
| expected | apparent MW |
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47 and 42 kDa (maize, tobacco, Chlamydomonas) |
| confirmed reactivity |
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Arabidopsis thaliana, Camelina sativa, Gossypium hirsutum, Gossypium barbadense, Nicotiana tabacum, Oryza sativa, Populus balsamifera, red sulfur bacterium Thiodictyon sp. Cad16 (isolated from Lake Cadagno) |
| predicted reactivity |
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dicots including: Gossypium mexicanum, Glycine max, Medicago sativa, Ricinus communis, Spinacia oleracea, Olea europea, Picea sitchensis, Vitis vinifera; monocots. Hordeum vulgare, Triticum aestivum, Zea mays, moss: Physcomitrella patens, green alga: Chlamydomonas reinhardtii |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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There are two forms of activase (alpha and beta) in some species (for example Arabidopsis, camelina, spinach, rice) and only one form in other species (tobacco, maize, Chlamydomonas). Alpha is about 46-47 Kda, beta is about 42 kDa. Species that have only one form have the beta form. |
| selected references |
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to be added when available, antibody released in November 2010. |
application example
 3, 5 and 11 μg of total soluble protein from Arabidopsis thaliana (1), Oryza sativa (2) and Camelina sativa (3) extracted with 50 mM Tricine-NaOH, pH 8, 10 mM EDTA, 1% PVP-40, 20 mM β-mercaptoethanol, 1 mM PMSF and 10 μM leupeptin were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 4% non-fat milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10,000 for over night with agitation. The antibody solution was decanted and the blot was rinsed briefly with H2O, then washed six times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG alkaline phosphatase conjugated, from Sigma-Aldrich) diluted to 1:3000 in 0.5% non-fat milk in TBS for 2h at RT with agitation. The blot was washed with four changes of TBS-T and developed for 5 min with NBT/BCIP according to the manufacturer’s instructions (Promega). There are two forms of activase (alpha and beta). Alpha is about 46-47 Kda, beta is about 42 kDa what is shown on the blot above.
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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