|
product information
|
| background |
|
Rubisco catalyzes the rate-limiting step of CO2 fixation in photosynthesis. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes. |
| immunogen |
|
KLH-conjugated synthetic peptide derived from all known sequences of RbcS from monocots and dicots including RuBisCO small subunit 1B of Arabidopsis thaliana At5g38430 |
| antibody format |
|
rabbit |
polyclonal |
|
serum |
lyophilized |
|
| quantity |
|
200 µl |
for reconstitution add 200 µl of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
AS03 037 | anti-RbcL rabbit antibody AS07 218 | Rubisco | 557 kDa hexadecamer AS09 409 | Rubisco quantitation kit |
| additional information |
|
to be added when available |
application information
|
| recommended dilution |
|
1: 5000 with standard ECL (WB) |
| expected | apparent MW |
|
20 | 15 kDa |
| confirmed reactivity |
|
Arabidopsis thaliana, Hordeum vulgare |
| predicted reactivity |
|
dicots including Glycine max, Nicotiana tabacum, monocots including Oryza sativa, Zea mays, liverwort Marchantia paleacea, green algae |
| not reactive in |
|
cyanobacteria |
| additional information |
|
to be added when available |
| selected references |
|
Sun et al. (2012). A dual-targeted purple acid phosphatase in Arabidopsis thaliana moderates carbon metabolism and its overexpression leads to faster plant growth and higher seed yield. New Phytol., January 24 (ahead of print). |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use LiveChat option in the left menu or contact us at support@agrisera.com
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
Save as PDF
|