SbtA | Sodium-dependent bicarbonate transporter
AS13 2657 | clonality: polyclonal | host: rabbit | reactivity: cyanobacteria
|Info:||More information||Product suggestions||Add review|
1 : 2000 with standard ECL (WB)
|Expected | apparent MW||
Cyanobium sp PCC7001,Synechochoccus elongatus PCC7942, Synechochoccus sp PCC7002, Synechocystis sp PCC6803
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
SbtA from Cyanobium PCC7001 is expected to be smaller on SDS-PAGE.
|Selected references||Holland et al. (2016). Impacts of genetically engineered alterations in carbon sink pathways on photosynthetic performance. Algal Research, 20 (2016) 87–99.|
Negative control, SbtA deletion strain (1), Synechochoccus elongatus PCC7942 (2,3), Cyanobium sp PCC7001 (4), Synechochoccus sp PCC7002 (5), Synechocystis sp PCC6803 (6) extracted with were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from ) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed using ATTOPhos.
Courtesy of Dr. Britta Forster, The Australian National University, Canberra, Australia
||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at email@example.com