Sec6 | Exocyst complex subunit

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AS13 2686  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana


27 st
Item No:
AS13 2686

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product information

Sec6 is a member of the exocyst complex involved in tethering vesicles to the plasma membrane during regulated or polarized secretion.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Sec6, UniProt F4IA34,  TAIR AT1G71820

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS13 2708 | Sec 8, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

88.4 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Carica papaya, Medicago truncatula, Ricinus communis
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information Antibody is also recognizing recombinant Sec6.
Selected references

Kulich et al. (2013). Arabidopsis exocyst subcomplex containing subunit EXO70B1 is involved in the autophagy-related transport to the vacuole. Traffic Aug14.

application example

western blot using anti-Sec6 antibodies

50 µg of total protein from Arabidopsis thaliana, wt whole plant extract of one week old seedlings (1), whole plant extract of one week old seedlings of Arabidopsis thaliana Col-0 expressing SEC6-GFP (2), whole plant extract of one week old seedlings of Arabidopsis thaliana sec8 k.o. mutant complemented by SEC8-GFP (3), extracted with SEC6/8 buffer (20mM HEPES, pH 6.8; 150mM NaCl; 1mM EDTA; 1mM DTT; 0.5% Tween 20) were separated on 10 % SDS-PAGE then using semi-dry transfer blotted 1h to the nitrocellulose membrane (1.5 mA/cm2). Blots were blocked with 5 % non fat milk in PBS overnight at 4°C with agitation. Blot was incubated in the primary antibody in 5 % milk in PBS at a dilution of 1: 7500 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed five times 5 min with PBST (PBS + 0.5% Tween 20) at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:15 000 in 5 % milk in PBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL (GE healthcare) according to the manufacturer's instructions. Exposure time was 10 seconds.

Courtesy of Dr. Michal Hála, UEB, Czech Republic

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