sll0528 | putative zinc metalloprotease sII0528 (100 ul)

361 €
AS10 1592 | clonality: polyclonal | host: rabbit | reactivity: cyanobacteria


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Item No:
AS10 1592

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product information
Background Sll0528 is a transmembrane metallo-protease from Synechocystis sp. PCC 6803. This protease belongs to SP2-type metallopeptidases, the key player of intra-membrane proteolysis.
Immunogen Synthetic peptide (amino acids 271 - 286) specific for protease Sll0528, Q55518 from Synechocystis sp. PCC 6803
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Liquid
Quantity 100 ĩl
Storage Short term 4°C. Long term -20°C. Repeated freezing and thawing is not recommended.  Solution contains 0.01% sodium azide.
Tested applications western blot (WB)
Related products

AS10 1623 | Anti-sll0528 | putative zinc metalloprotease sII0528, larger pack size

AS10 1596 | Anti-slr1821 | putative zinc metalloprotease slr1821

Secondary antibodies

Additional information Pre-immune serum for this product is available upon request.
application information
Recommended dilution 1: 2000 with standard ECL (WB)
Expected | apparent MW

40.5 kDa

Confirmed reactivity

Synechocystis sp. PCC 6803

Predicted reactivity Crocosphaera watsonii, Synechococcus, Microcystis aeruginosa, Nostoc sp.
Not reactive in no confirmed exceptions from predicted reactivity known at the moment
Additional information to be added when available
Selected references to be added when available

application example

western blot using anti-sll0528 antibodies Total protein from Synechocystis sp. PCC6803 were separated on 12% SDS-PAGE  and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with  5 % non-fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions.

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