SOC1 | Suppressor of constans overexpression 1

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS12 1856 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


14 st
Item No:
AS12 1856

Info: Product suggestions Add review
product information
Background SOC1 (Suppressor of constans overexpression 1) is a transcription activator active in flowering time control. Located in nucleus and cytoplasm. Widely expressed but not found in the apical meristem of short-day grown plants in vegetative stage.  Alternative names: AGAMOUS-LIKE 25, AGL25, FLC, FLF, FLOWERING LOCUS C, FLOWERING LOCUS F.
Immunogen KLH-conjugated synthetic peptidederived from Arabidpsis thaliana SOC1. UniProt: O64645,TAIR: AT2G45660
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.

Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS16 3160 | anti-CO | Constans, rabbit polyclonal antibody
AS12 1864 | anti-GI | Gigantea, rabbit polyclonal antibody
AS12 1864A | anti-GI | Gigantea, rabbit polyclonal antibody
AS12 2646 | anti-TOC1 | Timing of CAB expression 1, rabbit polyclonal antibody
AS14 2823 | anti-TOC1 | Timing of CAB expression 1 (other species), rabbit polyclonal antibody
AS06 198 | anti-FT/TSF | Flowering locus T and twin sister of FT, rabbit polyclonal antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1: 5000 (WB)
Expected | apparent MW

24 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity Brassica sp., Cardamine sylvatica, Sinapsis juncea
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references

to be added when available, antibody released in December 2013.

application example

2 µg of Arabidopsis thaliana total protein from 15 days old seedlings (1) from 200ng/μl extracted with buffer containing Tris (pH7.5), NaCl, Triton-x100 and protease inhibitors and yeast protein extracts from SG335 cells transformed with pGADT7/SOC1 construct (2) were separated on 12 % SDS-PAGE and blotted 40 min. to PVDF. Blots were blocked  with 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody  at a dilution of 1:5000 for 1.5h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation.  Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 45min at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 7 min. 

Double band in the yeast sample might be due to post-translational mofifications as experiment with yeast harbouring vector showed no cross-reactivity.

Courtesy of Dr Theoni Margaritopoulou, Agricultural University of Athens, Greece


western blot using anti-SOC1 antibodies

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at