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SPY | Spindly (100 ĩg)

195 €

AS13 2637 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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Item No:
AS13 2637

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product information
Background SPY (SPINDLY) is  O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway and circadian clock. It catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Acts as a repressor of GA signaling pathway to inhibit hypocotyl elongation. Alternative name: Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY.
Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SPY protein sequence, UniProt: Q96301, TAIR: At3g11540

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 2 x 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water to each tube.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies for signal transduction in plants

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 500 with ECL (WB)

Expected | apparent MW

101.4 kDa

Confirmed reactivity

recombinant SPY protein

Predicted reactivity Arabidopsis thaliana, Helianthus annus, Hordeum vulgare, Vitis vinifera, Glycine max, Oryza sativa, Brachypodium distachyon, Zea mays, Sorghum bicolor, Solanum lycopersicum, Physcomitrella patens
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

This antibody so far has been confirmed to detect recombinant SPY protein, GFP-tagged. 

Optimization of protein extraction and detection using most sensitive reagents may result in recognition of endogenous SPY.

Selected references

to be added when available, antibody released in March 2016. 


 


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