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Streptavidin HRP Conjugated, for ELISA

270 €
AS15 2860 | conjugate is suitable for the detection of biotinylated primary antibodies

PRODUCT INFORMATION IN PDF

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AS15 2860

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product information
Background

Streptavidin is a protein that has a very high affinity for biotin (known as vitamin B7 or H). The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is mostly used in molecular biology and bionanotechnology since the complex of streptavidin-biotin is resistant to organic solvents, denaturants etc. and extremes of temperature and pH.

Host
Clonality
Clone
Purity
Format
Quantity 1 mg
Reconstitution
Storage

Store freeze-dried (lyophilized) powder at 2-8 °C. Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Shelf life of this product is 1 year from date of receipt.

Tested applications This conjugate is designed to be used in the detection of biotinylated primary antibodies. It is suitable for use in ELISA, WB, IHC.
Related products
Additional information
application information
Recommended dilution The optimal working dilution should be determined by the investigator.
Suggested starting dilution(s):
  • 1:500-1:2 000 for Immunohistochemistry
  • 1:50 000-1:5 000 for ELISA/Western blot
Expected | apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information

Buffer composition: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % Kathon CG (v/v) is included as preservative.

Streptavidin is overexpressed in bacterial culture, purified and conjugated to horse radish peroxidase (HRP).

Selected references to be added when available, product released in March 2015.


||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com