Sub1C | submergence-1C

345 €

AS11 1770 | clonality: polyclonal | host: rabbit | reactivity:Oryza sativa

PRODUCT INFORMATION IN PDF

product information

background

Sub1C is a protein which is involved in rice tolerance to submergence.

immunogen

KLH-conjugated synthetic peptide derived from Oryza sativa Sub1C. Chosen peptide is convered in all 6 isoforms (C-1 to C-6).

antibody format

rabbit

polyclonal

serum

lyophilized

quantity

100 µl

for reconstitution add 100 µl, of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

AS11 1769 | Sub1A

additional information

to be added when available

application information
recommended dilution		1 : 5 000 with standard ECL (WB)
expected \| apparent MW		26 kDa
confirmed reactivity		Oryza sativa
predicted reactivity		Oryza sativa
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		to be added when available
selected references		to be added when available, antibody released in December 2011.

application example

Ten days old seedlings of Oryza sativa (cv Nipponbare) were submerged under water for 3 days, epigeal biomass was collected from control and treated samples for protein extraction. 25 µg of total protein were extracted with SDS Extraction Buffer (60mM Tris-HCl pH 8.0, 2 % SDS, 1,5 % Sucrose). Proteins were separated on 4-12 % SDS-PAGE (Criterion XT Precast Gel, Biorad) and blotted 7 min to PVDF with Trans-Blot Turbo Transferase System (Biorad). Blot was blocked with TTBS-milk for 1 h at RT with shaking. Blot was incubated in the primary antibody at a dilution of 1:5 000 overnight at 4 °C with shaking. The blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TTBS at RT with shaking. Blot was incubated in secondary antibody (goat anti-rabbit IgG HRP conjugated, AS09 602 Agrisera) diluted 1:20 000 in TTBS-milk for 45 min at RT with shaking. The blot was washed as above and developed for 5 min with ECL (LiteAblot Turbo, Euroclone) according to the manufacturers instructions. Total exposure time was 3 min. Images of the blot were obtained using BioSpectrum AC Imaging System (UVP). Arrow marks Sub1C protein.

Courtesy of Dr. Viet-The Ho, Scuola Superiore Sant'Anna, Italy

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