SVR4 | Supressor of variegation 4

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AS13 2725  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana, Hordeum vulgare



28 st
Item No:
AS13 2725

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product information

SVR4 (Supressor of variegation 4) is a nuclear-encoded chloroplast-located protein required for proper function of the plastid transcriptional machinery. Synonymes: ATECB1, EARLY CHLOROPLAST BIOGENESIS 1, ECB1, MESOPHYLL-CELL RNAI LIBRARY LINE 7, MRL7.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SVR4, UniProt Q9M0H2, TAIR: At4g28590

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS13 2726 | SVR4-like | Supressor of variegation 4 - like, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information

This protein is present in very low amounts only in early stages of plant development and this has to be taken into account when harvesting the tissue.

Western blots were done on: Arabidopsis thaliana total protein extract from cotyledons and Hordeum vulgare (2-14 days old plants).

application information
Recommended dilution

1 : 1000 - 1: 5000 with standard ECL (WB)

Expected | apparent MW

34.8 | 19 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare

Predicted reactivity please, inquire
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information
Selected references

Powikrowska et al. (2013). SVR4 (suppressor of variegation 4) and SVR4-like: two proteins with a rolein proper organization of the chloroplast genetic machinery. Physiol Plant. Sep 23. doi: 10.1111/ppl.12108.

application example

western blot using anti-SVR4 antibodies
SVR4 antibody tested on thylakoids isolated from 1 week old barley. Thylakoids were isolated from 1 week old  Hordeum vulgare plants. The sample corresponding to 3ug Chl was separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 10 min.

Courtesy of Dr. Małgorzata Powikrowska, University of Copenhagen, Danmark

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