SVR4 | Supressor of variegation 4
AS13 2725 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Hordeum vulgare
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1 : 1000 - 1: 5000 with standard ECL (WB)
|Expected | apparent MW||
34.8 | 19 kDa
Arabidopsis thaliana, Hordeum vulgare
|Predicted reactivity||please, inquire|
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
Powikrowska et al. (2013). SVR4 (suppressor of variegation 4) and SVR4-like: two proteins with a rolein proper organization of the chloroplast genetic machinery. Physiol Plant. Sep 23. doi: 10.1111/ppl.12108.
SVR4 antibody tested on thylakoids isolated from 1 week old barley. Thylakoids were isolated from 1 week old Hordeum vulgare plants. The sample corresponding to 3ug Chl was separated on 12% Criterion XT Bis-Tris SDS-PAGE (BioRad) gels and blotted for 25min 100V to PVDF membrane. Blot was blocked with 5% fat free skimmed milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight with agitation in 4˚C. The antibody solution was decanted and the blot was washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in secondary antibody (swine anti-rabbit IgG horse radish peroxidase conjugated, from Dako) diluted to 1: 5000 in 1% fat free skimmed milk in PBS-T for 1h at RT with agitation. The blot was washed 5 min in PBS-T and 1 min in PBS developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 10 min.
Courtesy of Dr. Małgorzata Powikrowska, University of Copenhagen, Danmark
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