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TNFa | Tumor necrosis factor alpha, Biotin conjugated

304 €

IMS06-102-325NB  |  clonality: polyclonal  |  host: hen  |  reactivity: human

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IMS06-102-325NB

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product information
Background

Tumor Necrosis Factor alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Immunogen

purified full length recombinant protein P01375

Host Hen
Clonality Polyclonal
Clone
Purity Affinity purified IgY
Format Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
Quantity 100 ĩl
Reconstitution
Storage

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB), ELISA (ELISA)
Related products

IMS01-142-310 | Etanercept (Enbrel®), recombinant human tumor necrosis factor receptor

Additional information

the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation;

Total IgY concentration for this antibody is 15 mg/ml

application information
Recommended dilution

1: 5000 with standard ECL (WB)

Expected | apparent MW

26 kDa

Confirmed reactivity

human

Predicted reactivity

cat, dog, horse, pig, primates

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

the antibody, in dilution 1:5 000, will detect 10 ng of SDS denatured and reduced human TNFa on western blots

Selected references

to be added when available



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