Plant/Algal cell antibodies / Photosynthesis / TOC/TIC

product information

Toc159 is located in the outer chloroplast membrane and part of of the Toc/TOC (translocon of the outer envelope membrane of chloroplasts) complex. It mediates the GTP-dependent initial stages of preprotein import into chloroplasts. In Arabidopsis thaliana, Toc159 is encoded by a small gene family: atTOC159, atTOC132, atTOC120, and atTOC90. Phylogenetic analysis suggested that at least two distinct Toc159 subtypes, characterized by atToc159 and atToc132/atToc120, exist in plants.

recombinant Toc159 (C-terminally His-tagged, including the central GTPase domain but lacking the N-terminal acidic domain as well as the C-terminal membrane associating domain) from Pisum sativum Toc159 protein sequence (Q9LKR1)

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunolocalization (IL)

AS06 150  |  anti-Toc75 (monocots and dicots)

AS08 292  |  anti-Toc75 (Pisum sativum)

Antibodies have been used in fluorescence and confocal microscopy and total internal reflectance confocal microscopy.

application information

1 : 10 000 with alkaline phosphatase (WB), 1: 500 (CM)

150 kDa (with protease inhibitors), 86 and 53 kDa (following proteolysis) and approx. 29 kDa degradation product (cross reaction with GTPase domain)

Pisum sativum

dicots, Oryza sativa

Arabidopsis thaliana

Toc159 is extremely sensitive to proteolytic processing in vivo therefore use of protease inhibitors is of crucial importance. 

to be added when available