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product information
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| background |
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Import of protein precurors into chloroplasts occurs via translocon complexes at the outer (Toc/TOC complex) and inner (Tic/TIC complex) envelope membrane. The major components of the Toc (TOC) complex in plants are Toc75, Toc34 and Toc159 (formerly Toc86). Toc75 (OEP75) forms the main import pore. It is an abundant protein in the outer envelope membrane of chloroplasts from expanding tissues but it is also found in plastids of roots, stems and flowers. Alternative names: 75 kDa translocon at the outer-envelope membrane of chloroplasts, import intermediate-associated protein of 75 kDa, 75 kDa chloroplast outer envelope protein, |
| immunogen |
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KLH-conjugated synthetic peptide derived from Toc75 protein sequence of Pisum sativum (Q43715) |
| antibody format |
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rabbit, |
polyclonal |
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serum, |
lyophilized |
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| quantity |
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200 µl |
- for reconstitution add 200 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB) |
| related products |
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AS06 150 | anti-Toc75 (monocots and dicots) AS07 239 | anti-Toc159 (guinea pig antibody)
AS07 236 | anti-Toc33 (hen antibody) |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1:1000 with standard ECL (WB) |
| expected | apparent MW |
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88 | 75 kDa (for Pisum sativum) |
| confirmed reactivity |
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Pisum sativum, Synechocystis PCC6803 |
| predicted reactivity |
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n.a. |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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to be added when available |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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