ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

TRF1 | Telomeric repeat-binding factor 1

285 €

AS16 3961  |  Clonality: Polyclonal  |  Host: Mouse  |  Reactivity: Mouse

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
9 st
Delivery:
Shipping:
Item No:
AS16 3961

Info: Product suggestions Add review
product information
Background

TRF1 (Telomeric repeat-binding factor 1) is a component of telesome which is involved in the regulation of telomere lenght and protection of chromosomes. TRF1 binds double-stranded 5'-TTAGGG-3' repeat on telomers and negatively regulates the lenght of telomers.

Immunogen

Full-length mouse TRF1.

Host Mouse
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized in 30% glycerol
Quantity 50 µl
Reconstitution For reconstitution add 35 ĩl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunofluorescence (IF), Western Blot (WB)
Related products

Antibodies for mammalian proteins

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 500 (IF), 1 : 2000 (WB)

Expected | apparent MW

48.2 kDa

Confirmed reactivity

Mus musculus

Predicted reactivity

Mammals including: human, rat, hamster, monkey

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment.

Additional information

To be added when available.

Selected references

To be added when available, antibody released in July 2016.


Application example:
Immunofluorescence using mouse anti-TRF1 antibody
Adult mouse testis spermatocytes were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100  for 10 minutes and blocked for 1 hour at room temperature with a blocking solution (3% BSA and 0.015% Triton X-100). Samples were then incubated with the mouse TRF1 serum (diluted 1:500 in blocking solution)  overnight at 4°C. An Alexa Fluor®594-conjugated mouse IgG was used at dilution at 1:500 as secondary antibody. Samples were sequentially stained with Rabbit SCP3 antibody to localize meiotic chromosomes.


||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at support@agrisera.com