UBQ11 | Ubiquitin
AS08 307 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, H. vulgare, N. benthamina, P.sativum, S. lycopersicum
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1: 10 000 with standard ECL (WB)
|Expected | apparent MW||
Arabidopsis thaliana, Hordeum vulgare, Nicotiana benthamina, Solanum lycopersicum
dicots including: Nicotiana tabacum, Glycine max, Glycine soja, Pisum sativum, Phaseolus vulgaris, Capsella rubella, Medicago truncatula, Vitis vinifera monocots including: Oryza sativa, Zea mays
|Not reactive in||
antibody can be used to check qubiquitination status in the whole plant extracts.
|Selected references||Moshe at al. (2015). Tomato plant cell death induced by inhibition of HSP90 is alleviated by Tomato yellow leaf curl virus infection. Mol Plant Pathol. 2015 May 12. doi: 10.1111/mpp.12275.
Hamorsky et al. (2015). N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential. Sci Rep. 2015 Jan 23;5:8003. doi: 10.1038/srep08003.
Kong et al. (2014). Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res. 2014 Apr 1.
Zulet et al. (2013). Proteolytic Pathways Induced by Herbicides That Inhibit Amino Acid Biosynthesis. PLoS ONE 8(9): e73847. doi:10.1371/journal.pone.0073847. (Pisum sativum, western blot)
Ferrández-Ayela et al. (2013). Arabidopsis TRANSCURVATA1 Encodes NUP58, a Component of the Nucleopore Central Channel. PLOS ONE, June 2013.
Ustun et al. (2013). The Xanthomonas campestris Type III Effector XopJ Targets the Host Cell Proteasome to Suppress Salicylic-Acid Mediated Plant Defence. PLOS Pathog. June 9.
5 μg of recombinant protein from Phytophora sp. (1), human (2), Arabidopsis thaliana His-tagged ubiquitin (3), Arabidopsis thaliana His-tagged SUMO protein (4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 10 000 anti-ubiquitin antibody (1h), followed by incubation with 1: 15 000 secondary anti-rabbit antibodies(1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL.
Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa.
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