UBQ11 | Ubiquitin

379 €

AS08 307  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, H. vulgare, N. benthamina, P.sativum, S. lycopersicum


2 st
Item No:
AS08 307

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product information

Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).


recombinant Arabidopsis thaliana ubq11, with N-terminal His-tag; UniProt:P0CH33, TAIR: AT4G05050

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products AS10 307A | antt-UBQ11 | Ubiquitin, affinity purified, rabbit antibody

AS10 307S | UBQ11 | Ubiquitin protein | positive control

Additional information
application information
Recommended dilution

1: 10 000 with standard ECL (WB)

Expected | apparent MW

8.5 kDa

Confirmed reactivity

Arabidopsis thaliana, Hordeum vulgare, Nicotiana benthamina, Solanum lycopersicum

Predicted reactivity

dicots including: Nicotiana tabacum, Glycine max, Glycine sojaPisum sativum, Phaseolus vulgaris, Capsella rubella, Medicago truncatula, Vitis vinifera monocots including: Oryza sativa, Zea mays  

Brassica napus, Medicago truncatula, Erythrane guttata, Brachypodium distachyon, Triticum aestivum, Cochliobolus heterostrophus, Solanum tuberosum, Solanum nigrum, Ipomoea batatas, Populus trichocarpa, Capsella rubella, Cymbidium faberi, Magnaporthe oryzae, Pyrus communis, Citrus sinensis, Citrus clementina, Eucalyptus grandis, Sorghum bicolor, Nicotiana tabacum, Ricinus communis, Theobroma cacao, Coffea canephora, Prunus persica,

Not reactive in


Additional information

antibody can be used to check qubiquitination status in the whole plant extracts.

Selected references Moshe at al. (2015). Tomato plant cell death induced by inhibition of HSP90 is alleviated by Tomato yellow leaf curl virus infection. Mol Plant Pathol. 2015 May 12. doi: 10.1111/mpp.12275.
Hamorsky et al. (2015). N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential. Sci Rep. 2015 Jan 23;5:8003. doi: 10.1038/srep08003.
et al. (2014). Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res. 2014 Apr 1.
et al. (2013). Proteolytic Pathways Induced by Herbicides That Inhibit Amino Acid Biosynthesis. PLoS ONE 8(9): e73847. doi:10.1371/journal.pone.0073847. (Pisum sativum, western blot)
Ferrández-Ayela et al. (2013). Arabidopsis TRANSCURVATA1 Encodes NUP58, a Component of the Nucleopore Central Channel. PLOS ONE, June 2013.
Ustun et al. (2013). The Xanthomonas campestris Type III Effector XopJ Targets the Host Cell Proteasome to Suppress Salicylic-Acid Mediated Plant Defence. PLOS Pathog. June 9.

application example


AS08 307 western blot ubiquitin.jpg


5 μg of recombinant protein from Phytophora sp. (1), human (2), Arabidopsis thaliana His-tagged ubiquitin (3), Arabidopsis thaliana His-tagged SUMO protein (4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 10 000 anti-ubiquitin antibody (1h), followed by incubation with 1: 15 000 secondary anti-rabbit antibodies(1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL.

Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa. 

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