UBQ11 | Ubiquitin
AS08 307 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, H. vulgare, N. benthamina, P.sativum, S. lycopersicum
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|Recommended dilution||1 : 10 000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Hordeum vulgare, Nicotiana benthamina, Solanum lycopersicum|
|Predicted reactivity||Brachypodium distachyon, Brassica napus, Capsella rubella, Cochliobolus heterostrophus, Citrus clementina, Citrus sinensis, Coffea canephora, Cymbidium faberi, Eucalyptus grandis, Erythrane guttata, Glycine max, Glycine soja, Ipomoea batatas, Medicago truncatula, Magnaporthe oryzae, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Populus trichocarpa, Prunus persica, Pyrus communis, Ricinus communis, Solanum nigrum, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Theobroma cacao, Zea mays , Vitis vinifera|
|Not reactive in||Algae|
|Additional information||Antibody can be used to check qubiquitination status in the whole plant extracts.|
|Selected references||Witzel et al. (2017). A Proteomic Approach Suggests Unbalanced Proteasome Functioning Induced by the Growth-Promoting Bacterium Kosakonia radicincitans in Arabidopsis. Front Plant Sci. 2017 Apr 26;8:661. doi: 10.3389/fpls.2017.00661.
Gorovits et al. (2017). The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation. Cell Stress Chaperones. 2017 Mar 21. doi: 10.1007/s12192-017-0766-0.
Moshe at al. (2015). Tomato plant cell death induced by inhibition of HSP90 is alleviated by Tomato yellow leaf curl virus infection. Mol Plant Pathol. 2015 May 12. doi: 10.1111/mpp.12275.
Hamorsky et al. (2015). N-Glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential. Sci Rep. 2015 Jan 23;5:8003. doi: 10.1038/srep08003.
Kong et al. (2014). Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res. 2014 Apr 1.
Zulet et al. (2013). Proteolytic Pathways Induced by Herbicides That Inhibit Amino Acid Biosynthesis. PLoS ONE 8(9): e73847. doi:10.1371/journal.pone.0073847. (Pisum sativum, western blot)
Ferrández-Ayela et al. (2013). Arabidopsis TRANSCURVATA1 Encodes NUP58, a Component of the Nucleopore Central Channel. PLOS ONE, June 2013.
Ustun et al. (2013). The Xanthomonas campestris Type III Effector XopJ Targets the Host Cell Proteasome to Suppress Salicylic-Acid Mediated Plant Defence. PLOS Pathog. June 9.
5 μg of recombinant protein from Phytophora sp. (1), human (2), Arabidopsis thaliana His-tagged ubiquitin (3), Arabidopsis thaliana His-tagged SUMO protein (4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 10 000 anti-ubiquitin antibody (1h), followed by incubation with 1: 15 000 secondary anti-rabbit antibodies(1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL.
Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa.
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