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product information
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| background |
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Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS). |
| immunogen |
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recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag; locus accession: At4g05050 |
| antibody format |
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rabbit; |
polyclonal; |
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serum; |
lyophilized |
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| quantity |
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100 µl |
- for reconstitution add 100 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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Western blot (WB) |
| related products |
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AS10 307S | UBQ11 | Ubiquitin protein | positive control |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1: 10 000 with standard ECL (WB) |
| expected | apparent MW |
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8.5 kDa |
| confirmed reactivity |
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Arabidopsis thaliana, Hordeum vulgare, Nicotiana benthamina, Solanum lycopersicum |
| predicted reactivity |
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dicots including: Nicotiana tabacum, Glycine max, Pisum sativum, monocots including: Hordeum vulgare, Oryza sativa, Zea mays |
| not reactive in |
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algae |
| additional information |
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antibody can be used to check qubiquitination status in the whole plant extracts. |
| selected references |
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to be added when available |
application example 5 μg of recombinant protein from Phytophora sp. (1), human (2), Arabidopsis thaliana His-tagged ubiquitin (3), Arabidopsis thaliana His-tagged SUMO protein (4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 10 000 anti-ubiquitin antibody (1h), followed by incubation with 1: 15 000 secondary anti-rabbit antibodies(1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL.
Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa.
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