UBQ11 | Ubiquitin, affinity purified antibody
AS08 307A | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, N. benthamina
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1: 10 000 with standard ECL (WB)
|Expected | apparent MW||
Arabidopsis thaliana, Nicotiana benthamina, Oryza sativa
dicots including: Nicotiana tabacum, Glycine max, Pisum sativum, monocots including: Hordeum vulgare, Oryza sativa, Zea mays
|Not reactive in||
antibody can be used to check qubiquitination status in the whole plant extracts.
|Selected references||to be added when available, antibody released in November 2013.|
10 μl of a total protein from Arabidopsis thaliana wt (20 μg of total protein) and Nicotiana benthamiana, was separated on 8% polyacralymide gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 5 000 anti-ubiquitin antibody (3h), followed by incubation with 1: 10 000 secondary anti-rabbit antibodies (1h) coupled with HRP and visualization (1 minute exposure) with standard ECL.
Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa.
Courtesy of Dr. Ari Sadanandom, University of Durham, UK
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