ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

UBQ11 | Ubiquitin protein (positive control)

96 €

AS10 307S | protein | positive control

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
1 st
Delivery:
Shipping:
Item No:
AS10 307S

Info: Product suggestions Add review
product information
Background

Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).

Source of positive control, ubiquitin protein: full length recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal 1XHis-3XHA-tag; locus accession: At4g05050

Host
Clonality
Clone
Purity
Format Liquid
Quantity 50 ĩl
Reconstitution
Storage

store protein standard at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

AS08 307 | UBQ11 | Ubiquitin antibodies

Additional information

Protein is provided in Laemli SDS loading buffer and should be heated up in 98°C for 5 minutes before loading on the gel.

application information
Recommended dilution

positive control: a 2.5-5 μl load per well is optimal for most chemiluminescent detection systems.

Expected | apparent MW

10-12 kDa

Confirmed reactivity
Predicted reactivity
Not reactive in

no confirmed exceptions from predicted reactivity currently known

Additional information

Concentration: 0.8 µg/µl. Recommended load per gel to allow Coomasie staining and western blot detection is 2.5-5 µl.

1x SDS Loading Buffer contains:

  • 50 mM Tris-HCl pH 6.8
  • 2% SDS
  • 10% glycerol
  • 1% β-mercaptoethanol
  • 12.5 mM EDTA
  • 0.02% bromophenol blue
Selected references

to be added when available


application example

 

 
 
5 μg of recombinant protein from Phytophora sp. (1), human (2), Arabidopsis thaliana His-tagged ubiquitin (3), Arabidopsis thaliana His-tagged SUMO protein (4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 10 000 anti-ubiquitin antibody (1h), followed by incubation with 1: 15 000 secondary anti-rabbit antibodies(1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL.
AS08 307 western blot ubiquitin.jpg
Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa.
 
 

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com