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V-ATPase | epsilon subunit of tonoplast H+ATPase (affinty purified)

371 €

AS09 577A clonality: polyclonal  |  host: goat  |  reactivity: higher plants includingA.thaliana, A.strigosa, M. truncatula, N. tabacum, S. lycopersicum

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AS09 577A

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product information
Background

Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Immunogen

KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS07 213 | V-ATPase | epsilon subunit of tonoplast H+ATPase rabbit antibodies

AS08 577 | V-ATPase | epsilon subunit of tonoplast H+ATPase goat antibodies, serum

collection of antibodies to membrane transport system

marker antibodies for plant cellular compartments

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000-1 : 3000 (WB)
Expected | apparent MW

26 | 31 kDa (Arabidopsis thaliana)

Confirmed reactivity Arabidopsis thaliana, Avena strigosa, Medicago truncatula, Nicotiana tabacum, Solanum lycopersicum
Predicted reactivity Algae, Chlamydomonas reinhardtii, Hordeum vulgare, Malus domestica, Mesembryanthemum sp., Oryza sativa,  Petunia sp.,Phaseolus sp. , Physcomitrella patens,  Pteris vittata (fern), Ricinus communis, Thellungiella sp., Zea mays, Vitis vinifera      Bull frog, Chicken, Bovine, Drosophila melanogaster, Human, Mouse, Rat
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.

2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.

Selected references

to be added when available. Antibody released in November 2009


application example

6 µg of total SDS-extracted protein from Avena strigosa roots (R) and leaves (L) , were separated on NuPage  LDS-PAGE 4-12% gradient acrylamide gel (Invitrogen) and blotted 1h to nitrocellulose. Filters were blocked 1h with 5% low-fat milk powder in TBS and probed with anti-V-ATPase antibodies (AS09 577 , 1:2000, 1h) and secondary anti-goat (1:5000, 1 h) antibody (Alexa 647) in TBS containing 5% low fat milk powder. Antibody incubations were followed by washings in TBS-T (containing 0.05% Tween-20, 0.1% Triton X-100) . All steps were performed at RT with agitation. Blots were scanned with a Typhoon scanner.

Courtesy Dr. Sam Mugford (JIC)

 

 

  western blot using goat and-V-ATPase antibodies

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