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V5

273 €
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AS15 3027  | clonality: monoclonal  |  host: mouse  |  reactivity: V5-tagged fusion proteins

PRODUCT INFORMATION IN PDF

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AS15 3027

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product information
Background V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.

Immunogen

KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide

Host Mouse
Clonality Monoclonal
Clone IgG1, clone V5.E10
Purity
Format Lyophilized in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.

Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), dot blot (Dot), ELISA (ELISA), immunoprecipitation (IP), immunohistochemistry (IHC)
Related products

AS15 3031 | V5 (mouse antibody)

AS15 2964 | V5, ALP conjugated (rabbit antibody)

AS15 2965 | V5, Biotin conjugated (rabbit antibody)


AS15 2966
| V5, DyLight® 488 conjugated (rabbit antibody)

AS15 2967 | V5, DyLight® 550 conjugated (rabbit antibody)

AS15 2968 | V5, DyLight® 650 conjugated (rabbit antibody)


AS15 2969 | V5, SureLight® R-Phycoerythrin conjugated (rabbit antibody)


other tag antibodies

Secondary antibodies

Additional information

exact working dilution for Dot Blot, ELISA and  IP needs to be determined experimentally

application information
Recommended dilution

1:1000-3000 with ECL (WB), 1:500-2000 (IHC)
 

Expected | apparent MW
Confirmed reactivity

V5-tagged fusion proteins

Predicted reactivity
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information Antibody is purified on Protein A.

For western blot incubate antibody for one hour at room temperature.
Selected references

to be added when available antibody available in October 2015



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