VDR1 | Violaxanthin de-epoxidase-related protein

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS12 2117 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


27 st
Item No:
AS12 2117

Info: More information Product suggestions Add review
product information

VDR1 (Violaxanthin de-epoxidase-related protein) is involved in a sterol biosythesis process and localized in a chloroplast.


part of recombinant Arabidopsis thaliana VDR1 protein sequence Q9SJ13, At2g21860, excluding membrane spanning part

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS12 2604 | anti-VDE1 | VIOLAXANTHIN DEEPOXIDASE 1 rabbit antibody

other antibodies to GreenCut proteins

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

59 | 52.86 kDa (minus a transit peptide)

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Medicago truncatula, , Populus balsamifera, Ricinus communis, Vitis vinifera

Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references

to be added when available, antibody released in April 2013.

application example

western blot using anti-VDR1 antibodies

From 0.1-1.2 µg of chlorophyll from Arabidopsis thaliana total leaf extract, extracted with buffer A (330 mM sorbitol, 25 mM Tricine pH 7.8, 1 mM EDTA, 10 mM KCl, 0,15 % BSA, 4 mM Na ascorbat (L-ascorbic acid), 7 mM L-cysteine) were separated on 15 % SDS-PAGE gel and blotted 1h to PVDF. Blots were blocked with 10% skimmed non fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 over night at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in TTBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 1-3 min.

Courtesy of Dr. Rikard Fristedt, UCLA, USA.

||| For other applications or usage on species other than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions please use the LiveChat option or contact us at