VDR1 | Violaxanthin de-epoxidase-related protein
AS12 2117 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1 : 1000 with standard ECL (WB)
|Expected | apparent MW||
59 | 52.86 kDa (minus a transit peptide)
|Confirmed reactivity||Arabidopsis thaliana|
Medicago truncatula, , Populus balsamifera, Ricinus communis, Vitis vinifera
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
to be added when available
to be added when available, antibody released in April 2013.
From 0.1-1.2 µg of chlorophyll from Arabidopsis thaliana total leaf extract, extracted with buffer A (330 mM sorbitol, 25 mM Tricine pH 7.8, 1 mM EDTA, 10 mM KCl, 0,15 % BSA, 4 mM Na ascorbat (L-ascorbic acid), 7 mM L-cysteine) were separated on 15 % SDS-PAGE gel and blotted 1h to PVDF. Blots were blocked with 10% skimmed non fat milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1 000 over night at 4ºC with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera) diluted to 1:10 000 in TTBS for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer’s instructions. Exposure time was 1-3 min.
Courtesy of Dr. Rikard Fristedt, UCLA, USA.
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