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VRN2 | Vernalization protein 2

356 €

AS08 341  |  clonality: monoclonal  |  host: mouse  |  reactivity: Triticum aestivum

PRODUCT INFORMATION IN PDF

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Item No:
AS08 341

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product information
Background

Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.

Immunogen

KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence

Host Mouse
Clonality Monoclonal
Clone
Purity Affinity purified
Format Lyophilized
Quantity 100 ĩg (2x50 ĩg)
Reconstitution
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), ELISA (ELISA)
Related products
Additional information

VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.

application information
Recommended dilution

1: 1000 (WB), 1: 10 000 (ELISA)

Expected | apparent MW

23.7 kDa

Confirmed reactivity

Triticum aestivum

Predicted reactivity

Triticum monococcum

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

there is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T. monococcum

Selected references

to be added when available



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