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XTH-Xet | XET5 Xyloglucan xyloglucosyl transferase

356 €

AS08 319  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Hordeum vulgare, Oryza sativa

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Item No:
AS08 319

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product information
Background

Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3;1,4)-β-d-glucans, are fundamental to cell wall properties.  These interactions have been assumed to be non-covalent in nature in most cases.  A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3;1,4)-β-d-glucans.  It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.

Immunogen

two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet 

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB) ELISA (ELISA)
Related products

AS05 080 | anti-Xet rabbit antibody

Additional information
application information
Recommended dilution

1: 500 with standard ECL (WB) , 1: 5000 (ELISA)

Expected | apparent MW

31.5 | 33 kDa

Confirmed reactivity

Hordeum vulgare, Oryza sativa

Predicted reactivity

monocots

Not reactive in

Zea mays

Additional information

to be added when available

Selected references Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.
Liu et al. (2013). Brittle Culm1, a COBRA-Like Protein, Functions in Cellulose Assembly through Binding Cellulose Microfibrils. PLoS Genet 9(8): e1003704. doi:10.1371/journal.pgen.1003704 (Oryza sativa, western blot)
Hrmova et al. (2007) A barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates and (1,3;1,4)-β. J. Biol. Chem. 82: 12951-12962.


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