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Antibody Production

For the last 30 years we have produced thousands of custom monoclonal and custom polyclonal antibodies for academic research worldwide as well as industry, diagnostics, and pharma. With our own animal facilities and immunological services in-house, we can offer antibody production for almost any research and scale needed. A list of chosen scientific references for antibodies produced at Agrisera can be found here.

Polyclonal and monoclonal antibodies

Both monoclonal and polyclonal antibodies can be used in variety of different applications including Western blot, ELISA, immunoprecipitation, immunofluorescence and immunohistochemistry and each have their own advantages and disadvantages which should be considered when deciding on choice of antibody.

Polyclonal antibodies are typically produced in rabbit, goat, hen or rat after activation of multiple B-cell clones. The polyclonal immune serum therefore contains a pool of antibodies with different chemical and physical properties to recognize multiple epitopes of the immunized antigen.

Advantages of recognizing multiple epitopes:

  • as the antibodies bind to different epitopes on a protein, a protein expressed in low levels can be easier to detect as the signal will be amplified
  • are more tolerant to small changes in the nature of the antigen, like polymerization or slight denaturation

General considerations:

  • are preferred when the nature of the protein is unknown
  • are preferred choice for detection of denatured proteins
  • have batch to batch variability between different animals
  • can recognize proteins with high homology. To ensure specificity the antigen sequence should be checked for significant cross reactivity
  • The immune response in animals immunized with the same antigen will be individually varying and therefore the general recommendations for polyclonal antibody production is to immunize at least two animals per antigen.

Monoclonal antibodies are typically produced from a single B-cell of immunized BalbC mice. By fusion of a B- and myeloma cell, a hybridoma cell line produces antibodies that recognize a single epitope with low cross reactivity.

Advantages of only recognizing one epitope of the antigen:

  • generally have low cross reactivity
  • facilitates standardization in manufacturing process such as diagnostics kits and clinical treatment

General considerations:

  • have the ability to produce unlimited amount of specific antibodies
  • have a high homogeneity
  • are preferred choice for detection of protein in tissues
  • are preferred choice in assays requiring quantification
  • may be too specific and thus loose ability to recognize the target in case of small changes of the antigen