Monoclonal antibodies
We offer a complete monoclonal antibody production service, consisting of the following stages:
Phase | Time (weeks) | Moment |
I | 1-3 | Design and peptide synthesis and coupling to KLH. |
I | 11 | Immunization and boost of 5 Balb/c mice. Evaluation of titre using ELISA. Serum will be sent to customer and priority of mice will be decided. |
II | 3-4 | Hybridoma fusion with the spleenocytes obtained from the two mice with the best titer, and myeloma cells. 2 fusions made in parallel are included. Supernatants from the hybridoma clones will be screened by ELISA and supernatants from up to 10 positive clones will be sent to customer's lab for evaluation. Shipment overseas at additional cost. NOTE! 30 days after the customer has received the supernatants for analysis, billing will begin for the remaining mice with 18 €/cage/week, unless otherwise agreed. |
III | 3 | The customer selects the clones of interest to proceed with. Subcloning of 3 clones is included. |
Each of the listed services from Phase I to Phase III can be offered separately.
Time schedule - approximately 4-8 months depending upon the immunogenic response to the antigen.
Scale-up and monoclonal antibody purification - from cell lines constructed at AgriSera or
supplied by the customer. Monoclonal antibodies secreted to the cell culture medium can be
subjected to further purification using Protein G or other system if required.
Total yields - small scale (up to 200 mg) and large scale (from 10 g upwards)
Confidentiality: All work done at AgriSera remains strictly confidential. If required a project
agreement can be signed.
