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Polyclonal Antibodies

The polyclonal antibody service at Agrisera includes peptide synthesis and conjugation, screening of pre immune serum and IgY, antibody production, purification and labeling. Polyclonal antibodies can also be raised against a customer supplied antigen. 

Needed amount of your protein                               

Amount of antigen for a standard immunization protocol: ~500 ug/animal. Preferable concentration: >1mg/ml in PBS, Tris or other suitable buffer. If the protein is present in gel the volume should be <1ml.

Species

In the process of producing polyclonal antibodies, choosing the right species is of crucial importance. At Agrisera we offer the possibility of selecting from a wide range of different species (rabbit, hen, goat and rat -or others on request). We will assist and give suggestions when making the final choice.                                      

Screening of pre immune serum or yolk

With Agrisera's pre-screening service you can test pre-immune samples and check that possible background signals are not detected in the molecular weight region of the protein under investigation.

Read more about pre screening

A test sample will be delivered after 11 weeks. After third immunization all serum and yolk samples are checked for the presence of antigen specific antibodies using ELISA.

Immunization schedule

	Goat (IgG)	Rabbit (IgG)	Hen (IgY)
Time schedule [weeks]	15	15	12
Pre immune	5 ml serum	3 ml serum	<50 mg of total IgY
Immunizations	4	4	3
Antigen [µg/immunization]	20-500	20-500	20-500
Immune product	80-100ml serum	80-100ml serum	<0,4 g of purified IgY

 

 

 

 

 

 

 

 

 

 

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