IgY purification methods
Bulk precipitation methods:
- ammonium sulfate precipitation (Schwarzkopf and Thiele 1996)
- dextran sulfate precipitation (Jensenius 1981)
- polyethylene glycol (PEG) precipitation, video tutorial from Journal of Visualized Experiments
A waste disposal should be considered when using methods below:
- caprylic acid extraction (McLaren et al. 1994)
- chloroform extraction/PEG combined extraction (Polson 1990)
- propanol-aceton extraction (Bade and Stegemann 1984)
Commercially available kits and matrixes (different capacity to process egg yolk):
- EGGstractTM kit (Promega)
- EggcellentTM Chicken IgY Purification Kit (Pierce)
- IMSORB Anti IgY (Biotez)
- HiTrap® IgY purification (GE Healthcare)
Industrial scale methods
- Column and ultrafiltration method (Kim and Nakai 1996, 1998)
Recommended Literature
- This manual contains detailed protocols of IgY purification by different methods as well as trouble shooting.
"Chicken Egg Yolk Antibodies, Production and Application" R. Schade et al.2000, Springer-Verlag, Lab Manuals, ISBN 3-540-66679-6. - "Isolation and Purification of Chicken Egg Yolk immunoglobulins: A review" B. De Meulenaer and A. Huyghebaert 2001, Food and Agricultural Immunology, 13:275-288.
- "IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation. Paulty et al. (2011). Journal of Visualized Experiments.
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