Monoclonal antibodies - from small to large-scale production.
We offer a complete monoclonal antibody production service, consisting of
the following stages:
-
Stage I
Antigen preparation (AgriSera will give advice and synthesise
peptides if required).
Immunisation of at least four Balb/c mice. Initial screening of serum and
its evaluation by ELISA. Serum samples can be send to the customer
for testing, if requested.
- Stage II
Fusion of spleen cells from two animals with the best response
against the antigen to SP2/0 cells. Screening of positive clones using ELISA,
followed by their cultivation and freezing.
- Stage III
Subcloning of selected positive, antibody secreting lines by
limiting dilution (few subclones/line). Expansion of chosen clones, freezing and delivery to the
customer.
Important note - each of the listed services from Stage I to Stage III can
be offered separately.
Time schedule - approximately 4-8 months depending upon the immunogenic
response to the antigen.
Scale-up and monoclonal antibody purification - from cell lines
constructed at AgriSera or supplied by the customer. Monoclonal antibodies
secreted to the cell culture medium can be subjected to further
purification using Protein G or other system if required.
Total yields - small scale (up to 200 mg) and large scale (from 10 g upwards)
Special service on request:
Purification of problematic monoclonal antibodies - for example IgG3 - including optimisation of
storage conditions. Optimisation of purification conditions for unstable and aggregating monoclonal
antibodies. Please, inquire.
Confidentiality: All work done at AgriSera remains strictly confidential.
If required project agreement can be signed.
Excellent reference about working with monoclonal antibodies/monoclonal antibody production:
"Monoclonal antibodies: principles and practice" by James W. Goding, 1996,
ISBN 0-12-287023-9; Publisher: Academic Press.