Monoclonal antibodies - from small to large-scale production.

We offer a complete monoclonal antibody production service, consisting of the following stages:

Stage I
Antigen preparation (AgriSera will give advice and synthesise peptides if required). Immunisation of at least four Balb/c mice. Initial screening of serum and its evaluation by ELISA. Serum samples can be send to the customer for testing, if requested.
Stage II
Fusion of spleen cells from two animals with the best response against the antigen to SP2/0 cells. Screening of positive clones using ELISA, followed by their cultivation and freezing.
Stage III
Subcloning of selected positive, antibody secreting lines by limiting dilution (few subclones/line). Expansion of chosen clones, freezing and delivery to the customer.

Important note - each of the listed services from Stage I to Stage III can be offered separately.

Time schedule - approximately 4-8 months depending upon the immunogenic response to the antigen.

Scale-up and monoclonal antibody purification - from cell lines constructed at AgriSera or supplied by the customer. Monoclonal antibodies secreted to the cell culture medium can be subjected to further purification using Protein G or other system if required.
Total yields - small scale (up to 200 mg) and large scale (from 10 g upwards)

Special service on request: Purification of problematic monoclonal antibodies - for example IgG3 - including optimisation of storage conditions. Optimisation of purification conditions for unstable and aggregating monoclonal antibodies. Please, inquire.

Confidentiality: All work done at AgriSera remains strictly confidential. If required project agreement can be signed.

For price estimation of your project please - inquire.

Excellent reference about working with monoclonal antibodies/monoclonal antibody production:
"Monoclonal antibodies: principles and practice" by James W. Goding, 1996,
ISBN 0-12-287023-9; Publisher: Academic Press.

Last changed: 2009-04-02 by WebMaster