by
experience
Polyclonal antibody production program dictated by customer's requirements
What kind(s) of antigen(s) can be injected?
Which species to choose?
Examples of chosen immunisation protocols performed at AgriSera
| Goat (IgG) | Rabbit (IgG) | Hen (IgY) | |
|---|---|---|---|
| Time schedule [weeks] | 14 | 14 | 11 |
| Pre immune | 5 ml serum | 3 ml serum | up to 50 mg of total IgY |
| Immunisations | 4 | 4 | 3 |
| Antigen*[µg/immunisation] | 20-500 | 20-500 | 20-500 |
| Immune product | 250 ml serum | 80-130 ml serum | up to 0,4 g of purified, total IgY |
Screening of pre-immune serum and yolk is recommended in following cases:
- future work is going to be done on bacterial extracts
- future work is going to be done on extracts from maize, corn, wheat or other plants which are
included in animal diet
- independent confirmation that expected target protein is detected
- antibody pools with distinctive properties, complementing each other in different
techniques
- double staining possible
- The antigen is a conserved mammalian protein.
- Interference in immunological assays needs to be avoided.
- Economic production of large amounts of antibodies (grams) is required.
- Antibody production failed using a mammalian system.
In general it is good (if possible) to check that possible background singal is not detected in the
molecular weight region of a protein under investigation. Important is a future signal to noise ratio.
Time schedule - first antibody sample can be delivered after 5-8 weeks from a first
immunisation in the form of serum, egg yolk or
purified immunoglobulins.
Antibody titre control - after third immunisation all serum or egg yolk samples are routinely checked
for the presence of antigen specific antibodies using ELISA test.
We can also help you to setup up your own ELISA assay.
EGGS:
Eggs from immunised hens will be routinely stored at AgriSera for two months
after specific project is finished.
LARGE SCALE:
AgriSera has the necessary knowledge and experience for a large scale antibody
production in hens-IgY (grams to kilgorams of antibodies/year). Scale of production
can fulfill demands of functional food projects.
Advantages of using both IgG (rabbit, goat) and IgY (hen) antibodies developed against the same antigen:
AgriSera also recommends hens for antibody production in cases like:
AgriSera has successfully produced antibodies in hens against antigens from plants, bacteria, Archea, insects, and mammals.
Confidentiality: All work done at AgriSera remains strictly confidential. If required project agreement can be signed.
Price estimates: for specific project are available on request.
Last changed: 2009-04-02 by WebMaster