Bulk precipitation methods:

• ammonium sulfate precipitation (Schwarzkopf and Thiele 1996)
  
  
• dextran sulfate precipitation (Jensenius 1981)
  
  
• polyethylene glycol (PEG) precipitation (Polson and Wechmar 1980)
  
  

• caprylic acid extraction (McLaren et al. 1994)
  
  
• chloroform extraction/PEG combined extraction (Polson 1990)
  
  
• propanol-aceton extraction (Bade and Stegemann 1984)
  
  

• EGGstractTM kit (Promega)
  
  
• EggcellentTM Chicken IgY Purification Kit (Pierce)
  
  
• IMSORB Anti IgY (Biotez)
  
  
• HiTrap® IgY purification (Amersham Pharmacia Biotech)
  
  

• Column and ultrafiltration method (Kim and Nakai 1996, 1998)
  
  

• This manual contains detailed protocols of IgY purification by different methods as well as trouble shooting.
  "Chicken Egg Yolk Antibodies, Production and Application" R. Schade et al.2000, Springer-Verlag, Lab Manuals, ISBN 3-540-66679-6.
  
  
• "Isolation and Purification of Chicken Egg Yolk immunoglobulins: A review" B. De Meulenaer and A. Huyghebaert 2001, Food and Agricultural Immunology, 13:275-288.