AGO1 | argonaute 1 (40 ĩg, ALP-conjugated)

406 €

AS09 527-ALP | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana


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AS09 527-ALP

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product information

AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

This antibody is directly conjugated to Alkaline Phosphatase (ALP).


N-terminal peptide of Arabidopsis thaliana AGO1 UniProt:O04379, TAIR: At1g48410.

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4.
Format Liquid, conjugated to ALP.
Quantity 40 ĩg

Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Tested applications Western blot (WB)
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collection of antibodies to micro RNA

Plant protein extraction buffer

Secondary antibodies

Additional information

Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.

application information
Recommended dilution

1: 1000 (WB)

Expected | apparent MW

116.4 | 130 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera

Not reactive in

Chlamydomonas reinhardtii

Additional information

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

TCA acetone precipitation method

Selected references To be added when available, antibody released in November 2015.

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