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GID2 | F-BOX protein GID2 (SLEEPY 1)
AS13 2638 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana GID2 sequence, UniProt: Q9STX3, TAIR: At4g24210
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 5000 (WB) Expected | apparent MW: 17.4 | 18 kDa (Arabidopsis)
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Brassica napus, Medicago truncatula, Glycine max, Ricinus communis, Populus trichocarpa, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: Marchantia polymorpha - Application Examples
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Application example 
15µg of total protein from inflorescence of Col0, spy mutant, and 35S-SPY extracted with extraction buffer (DTT 100uM, Tris pH 6,8 67,5mM, Urea 4M, SDS 3%,glycerol 3% and bromophenol 0,1%) were separated on 10% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody diluted to 1:10 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent, according to the manufacturer's instructions. Exposure time was 30 seconds.
Courtesy Dr. Patrick Achard, CNRS, France - Background
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Background: GID2 | F-BOX protein GID2 (SLEEPY 1) is an essential component of the SCF-type E3 ligase complex, which positively regulates the gibberellin signaling pathway. It mediates the ubiquitination and subsequent degradation of DELLA proteins (GAI, RGA and RGL2). Localized in nucleus and expressed in all tissues tested so far.
- Product Citations
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Selected references: Ji et al. (2023). Evolution of a plant growth-regulatory protein interaction specificity. Nat Plants. 2023 Dec;9(12):2059-2070.doi: 10.1038/s41477-023-01556-0. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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Accessories
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