Rhodanese/cell cycle control phosphatase superfamily protein

Brassica napus, Gossypium arboreum 

Application example

10, 15, 20 µg of total protein from Arabidopsis thaliana chloroplast and thylakoids were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose. Blots were blocked with 10% Non fat dairy milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was washed twice, then washed three times for 15 minutes in TBS-T at RT weith agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in TBST for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 1 minute.

Courtesy of Dr. Rikard Fristedt VU University Amsterdam, The Netherlands

Rhodanese/cell cycle control phosphatase superfamily protein is localized in chloroplasts.