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ACT | Actin (monoclonal, clone mAbGPa 10-B3)
AS16 3141 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Dictyostelium discoideum
- Product Info
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Immunogen: Full length recombinant Arabidopsis thaliana Actin-8 (ACT8) expressed in E.coli, Uniprot: Q96293 Sub class: IgG2b Host: Mouse Clonality: Monoclonal Purity: Immunoglobulin IgG2b, Protein G purified in 0.1M Sodium Phosphate, pH 7.4. Contains 0.15M NaCl, 0.05% (w/v) sodium azide. Format: Liquid Quantity: 100 µg Storage: Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1-2 ug/ml (WB) Expected | apparent MW: 45 | 45 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Dictyostelium discoideum Predicted reactivity: Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Camelina sativa, Camellia lipoensis, Cucumis sativus, Dendrocalamus latiflorus, Dionaea muscipula, Gossypium sp., Eucalyptus grandis, Euphorbia lathyris, Gynura bicolor, Ficus microcarpa, Haloxylon ammodendron, Helianthus annuus, Hevea brasiliensis, Lilium regale, Medicago sativa, Mimosa pudica, Nitraria sibirica, Oryza sativa subsp. japonica, Oryza sativa subsp. indica, Oxytropis ochrocephala, Paeonia lactiflora, Panax notoginseng, Populus trichocarpa, Prunus avium, Ricinus communis, Striga asiatica, Theobroma cacao, Torenia fournieri, Trifolium pRatense, Ziziphus jujuba
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known. - Additional Information
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Additional information: This antibody is purified on a protein G column. It recognizes Arabidopsis actins ACT1, 2, 3, 4, 7, 8, 11, 12 and Dictyostelium actin
- Background
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Background: Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. - Product Citations
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Selected references: Sultan et al. (2017). The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016 Nov;28(11):2805-2829.
Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281 - Reviews:
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Accessories
AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stoloniferacv. ‘Penncross’,Arabidopsis thaliana, Brassica sp., Cannabis sativa L., Cucumis sativus, Cynara cardunculus, Glycine max, Hordeum vulgare, Nicotiana tabacum, Phaseolus vulgaris, Phoenix dactylifera, Picrorhiza kurroa, Setaria italica, Solanum tuberosum, Triticum aestivum, Zea mays
AS09 627 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Mouse IgG (H&L)
AS16 3141 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana, Dictyostelium discoideum