Agrisera blog

Detecte and quantify Rubisco using antibodies with 20 years of publication record

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous, from purple bacteria to flowering plants, and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

There are the following advantages of using Agrisera antibodies to Rubisco:

Working in very high dilutions, 1: 10 000 1h/RT incubation
Broad species reactivity, including higher plants, mosses, algae, cyanobacteria and diatoms. One and the same antibody will detect and quantify Rubisco in thousands of species
Suitable for Western blot, immunolocalization and tissue printing
Possibility of quantification in quantitative Western blot and ELISA
Cited in hundreds of publications, over the last 20 years

In the recent publication by Nuamzanei et al. (2024). "Impact of polyvinyl chloride (PVC) microplastic on growth, photosynthesis and nutrient uptake of Solanum lycopersicum L. (Tomato)", was investigated, using anti-RbcL antibodies from Agrisera.

Explore and pick up suitable antibodies or kits for the detection of the most abundant protein on Earth, Rubisco.

For images of Rubisco, and schemes in which this protein is involved, check out our free images for download here.

Which secondary antibody to choose for Western blot for protein detection in plant and algal samples?

A matching secondary antibody, that can be used at high dilutions, will contribute to good Western blot results, with less risk for background signal. Beyond this, it can be used for many experiments, and is therefore very cost-efficient. The differences between secondary antibodies from various suppliers are discussed here.

Agrisera's primary antibodies are produced in chicken, goat, mouse, rabbit and rat. The following high-titer secondary antibodies are appropriate matches:

Anti-Chicken IgY (AS10 1489)
Anti-Goat IgG (AS09 605)
Anti-Mouse IgG (AS09 627)
Anti-Rabbit IgG (AS09 602)*
Anti-Rat IgG (AS10 1187)

At the bottom of each primary antibody product page, you will find a recommended secondary antibody to use with it.

*This antibody is in the group of the most published antibodies in the world, based on publication record analyses performed by CiteAB.

If you need advice on the choice of the most suitable secondary antibody for your experiments, please ask us, either through the chat on Agrisera website, or by email.

Study Photosystem I with Agrisera comprehensive antibody set

Over the last 20 years, Agrisera has developed antibodies to study Photosystem I (PSI). This protein complex plays a central role in the conversion of light energy into chemical energy, in the form of ATP and NADPH. This energy is then used to power the biochemical reactions of photosynthesis, and ultimately produce carbohydrates. Agrisera antibodies specifically targeting PSI components can help accurately identify and quantify these proteins in plant, algal and diatom samples. This enables comparative studies, and facilitates the understanding of photosynthetic processes across a wide taxonomic range.

Agrisera PSI antibodies can be applied in:

• Western blotting to detect the presence of PSI subunits in plant, algae and diatom samples, which will help in understanding the expression levels of PSI proteins under different experimental conditions.

• Immunofluorescence and Immunohistochemistry to visualize the spatial distribution of PSI within plant and algal cells to study the subcellular localization of PSI and its dynamics under various physiological conditions.

• Co-immunoprecipitation (Co-IP) to identify protein-protein interactions involving PSI subunits or associated proteins. to determine the molecular interactions and protein complexes involved in PSI function and regulation.

Agrisera PSI antibodies will help determine the impact of genetic mutations on PSI function and assembly.

Browse the full collection

10% discount in return for feedback

Agrisera offers a 10% discount on any product from our catalog, in return for a short note left on the specific product page.

Example of product reviews:

To leave a review:

Go to the product page for an Agrisera antibody or reagent you have previously used
Find the tab "Reviews"
Click on "Leave a review"
Write your short comment, including details like: type of extract, technique, dilution and overall result obtained with this product
Save your comment

Once the review is submitted, contact us at [email protected] and you will be granted a 10% discount on your next order.

Thank you!

Agrisera supports Eastern Regional Photosynthesis Conference 2024

Agrisera supports the Eastern Regional Photosynthesis Conference 2024, held between 12th and 14th of April, at The Marine Biological Laboratory (MBL) in Woods Hole, MA, USA.

The conference program will includes the following topics:

• Mechanism of Light Harvesting and Regulation
• Light Driven Reactions
• Carbon Fixation and Concentration
• Evolution and Applications of Photosynthesis

Agrisera Educational Posters, focused on photosynthesis, will be available as hard copies for the meeting participants. Agrisera will also conduct an online quiz, with the first prize being the book Photosynthesis: Solar Energy for Life, by Dr. Dmitry Shevela, Prof. Lars Olof Björn, Prof. Govindjee.

The above image, and many other images and schemes by Scigrafik and Agrisera, can be downloaded for free here.

Most published photosynthetic antibodies, 20 % off until end of June 2024

Agrisera's antibody collection to proteins involved in photosynthesis has been developed over the last 20 years, in close collaboration with researches in the field, from all around the world.

The collection contains antibodies targeting proteins from the following groups:

ATPsynthase | Carotenoid metabolism | Chaperones/Transporters | Chloroplast biogenesis/architecture | Chlorophyll | Green Cut | LHC | Light acclimation | Kinases | PSI (Photosystem I) | PSII (Photosystem II) | Proteases | Protein standards-quantitation | Rubisco/Carbon metabolism | Sucrose metabolism

Each year, hundreds of publications are published, using these antibodies, which makes them the most used photosynthetic antibodies on the market. Agrisera antibodies place 31st of over 250 catalog antibody suppliers, based on publication record, not limited to plant science.

For 20% discount for antibodies to proteins involved in photosynthesis, use promotional code Photo20. Valid until end of June 2024.

Needed antibody not found in our collection? Contact us!

Agrisera online Western blot workshop for researchers in Taiwan, part I

Agrisera Western blot workshop for researchers in Taiwan, part I

Versatile ECL reagent set, which can save your day!

Question: If something goes wrong with the development of a Western blot with ECL, or if I have too high background or too low signal, can I redo the reaction on the same blot?

Answer: Yes! Within the same day.
Simply wash the membrane with the appropriate washing buffer, used in your protocol, 1-2 times for 5-10 minutes, and apply another one of the ECL reagents from the Agrisera ECL reagent set. If there is too much background, and a very strong band for the target protein, apply Agrisera ECL Bright. If a target protein band is not detected, apply Agrisera ECL SuperBright.

If you want to learn more about how to improve your Western blot, click here.

Agrisera's versatile ECL set of 2 reagents with different sensitivity

Blot A shows an abundant photosynthetic protein, PsbA, visualized with too sensitive of a reagent, which was washed away, and the membrane was subsequently developed with less sensitive Agrisera ECL Bright (Blot B).

Take advantage of Agrisera antibodies to proteins involved in response to plant pathogen attack

As plants cannot move and change their habitat quickly as animals can, they have instead developed other amazing strategies to cope with various pathogen attacks. Listed below are some examples of plant pathogens.

Fungal diseases: These pathogens typically thrive in warm, moist conditions and can infect various parts of the plant, such as leaves, stems, fruits, and roots.
Bacterial diseases: Bacterial pathogens infect plants through wounds or natural openings, causing diseases like bacterial wilt, leaf spot, and soft rot, and can spread rapidly through the whole plant.
Viral diseases: Viruses can infect plants through vectors such as insects, nematodes, and even human activities, like grafting. Viral diseases can significantly reduce crop yields and quality.
Nematode damage: Nematodes are microscopic roundworms that feed on plant roots, leading to reduced nutrient uptake and stunted growth. Root-knot nematodes, for example, cause the formation of galls on roots, interfering with water and nutrient absorption.

All antibodies to proteins involved in environmental stress response are offered at a 20% discount until the end of March. Use discount code Stress20.

Agrisera Antibodies to proteins involved in response to pathogen attack

Agrisera supports Jan Andersson Award of the Australian Society of Plant Scientists

Agrisera is sponsoring this year's Jan Anderson Award, granted by the Australian Society of Plant Scientists.

Joan (Jan) Mary Anderson was an adjunct professor at the Australian National University. She received an honorary doctorate at Umeå University (Sweden) in 1998. She was an internationally acclaimed plant biologist, renowned for her discoveries on the organization of the intricate membrane structures that carry out solar energy conversion in cells of green plants. Her work was the first to show that the photosynthetic mechanisms consists of two fundamental components; Photosystem I and Photosystem II. Her research career spanned over 55 years, mostly at the Division of Plant Industry CSIRO Canberra (1961-97), and later at the Research School of Biological Sciences at the Australian National University.

Jan was a passionate and creative female scientist who was well ahead of her time. She was an inspiration to young researchers, for whom her enthusiasm and curiosity were infectious. Professor Jan Anderson died on 28 August 2015 after a short illness. In recognition of her life and achievements in photosynthesis research as a pioneering female scientist, this award will acknowledge talented female plant science researchers.

Full description of the award

Agrisera supported the 37th Molecular Biology of Plants meeting in Germany

The 37th Molecular Biology of Plants conference, organized by the Section Plant Physiology and Molecular Biology of the Germany Society for Plant Sciences, took place the 4th-7th of March, North Rhine-Westphalia, Germany. The organizers were Prof. Dr. Christopher Grefen (Bochum), Prof. Dr. Ute Höcker (Cologne), and Prof. Dr. Andreas Meyer (Bonn).

Agrisera supported the meeting with The Best Poster Prize, awarded to Dr. Marc Somssich from the Max Planck Institute for Plant Breeding Research in Cologne. The winning poster title was "Spatially resolved immune responses of Arabidopsis thaliana to colonization by Fusarium oxysporum".

Participants could also enjoy hard copies of Agrisera's Educational Posters, covering topics such as photosynthesis and epigenetics, and developed with scientists in these fields.

Winner of Agrisera Best Poster Prize 2024

Left to right: Prof. Ute Hoecker and Dr. Marc Somssich. Courtesy of Dr. José Ugalde from the University of Bonn.

Wide species range of blocking serum for ELISA and immunolocalization

For most immunoassays, like immunolocalization, ELISA or Western blot, blocking is a crucial step. This procedure blocks spaces that are not occupied by immobilized proteins, and thus prevents non-specific binding of the antibodies used in the assay. If blocking is not performed, false signals or high background will occur.

If normal serum is used as a blocker, it is recommended to use serum derived from the same host species as the detection antibody, conjugated with a reporter enzyme, like HRP (Horseradish peroxidase) and ALP (Alkaline phosphatase), or a fluorochrome, like DyLight®, FITC or others.

Example:
Primary antibody: Rabbit anti-target protein

Blocking serum: Normal Chicken Serum (usually used at 5% v/v in PBS with Tween®20 detergent)

Secondary antibody: Chicken anti-Rabbit IgG-HRP conjugated

Note: Rabbit serum cannot be used for blocking in such a combination.

Agrisera blocking serum from various species, is provided in three purity grades: non-purified, purified on Protein G and ultrapure, available as in research and bulk quantities.

Not certain which blocker to use? Ask Agrisera.

Plant oxidative stress antibody set, 20 % off until end of March

Oxidative stress response in plants involves a complex network of proteins that work together to mitigate the damage caused by reactive oxygen species (ROS), such as superoxide radicals, hydrogen peroxide, and hydroxyl radicals. Below is a list of the main proteins involved in this process:

Superoxide dismutase (SOD): Enzyme that catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide. There are different isoforms of SOD found in various cellular compartments, such as cytosol, mitochondria and chloroplast.
Catalase (CAT): Enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen. It is primarily located in peroxisomes and helps neutralize excess hydrogen peroxide, generated during oxidative stress.
Ascorbate peroxidase (APX): Enzyme that uses ascorbate as a reducing agent to scavenge hydrogen peroxide and convert it into water, while oxidizing ascorbate to monodehydroascorbate. This enzyme is crucial for maintaining the redox balance in chloroplasts and other cellular compartments.
Glutathione peroxidase (GPX): Enzyme that utilizes glutathione (GSH) as a cofactor to reduce hydrogen peroxide and lipid hydroperoxides to their corresponding alcohols. It plays a significant role in protecting cells from oxidative damage by ROS.
Glutathione S-transferases (GSTs): Family of enzymes that catalyze the conjugation of glutathione to a wide range of electrophilic compounds, like lipid peroxides and xenobiotics. They are involved in both detoxification processes and in the regulation of cellular redox status.

Choose 5 specific antibodies, combined according to your requirements from the following antibodies:

APX | Cat | Cu/ZnSOD | FeSOD | HRP | Gamma-ECS | GR | GS | MnSOD | RBOHD

Offered at 20% discount until the end of March, with the promotion code Stress20.

The set also includeds a high-titer secondary Goat anti-Rabbit antibodies, HRP conjugated and Agrisera ECLBright detection reagent.

Online Western blot workshop for Australia and New Zealand with Agrisera distributor, Sapphire Bioscience

Western blot issues?

Get them resolved and learn many new tips on how to improve your results, in an online Western blot workshop, organized by Agrisera and Sapphire Bioscience, Agrisera's distributor in Australia and New Zealand.

Date: Wednesday, March 20th, 3-5 PM (AEDT).
Request the link and send your questions here.

Each participant will receive:

New knowledge
Possibility to win a useful prize
Link to the workshop recording
Pdf of the presentation with links
Certificate of participation

Read the feedback from participants of previous workshops here and join us on the 20th of March!

Online Western blot workshop with Sapphire Biosciences

Help the plant community and get the chance to win a free antibody!

Help the plant community!

Share your protein extraction protocols to assist Agrisera in creating a new informative poster on this topic, which is going to be the 8th poster in Agrisera Educational Poster series.

By participating, you’ll also get a chance to win an antibody of your choice. Complete this quick survey here.

Agrisera at Auxentric meeting at SLU in Ume�

Between the 27th and 29th of February, Agrisera attended The Auxentric Meeting, organized by Professor Stéphanie Robert, at UPSC. The meeting was held at SLU's facilities in Umeå.

Researchers from institutions around Europe were gathered at this meeting, focused on auxin transport, auxin involvement in plant development and cell shape aquisition.

Agrisera Best Talk Prize was awarded to Mark Bal from John Innes Centre, the UK, for the talk "Two auxins are better than one: Duplicated auxins in pea fruit development".

Thank you to everyone who came by Agrisera's table, for the nice chats and interesting discussions!

Left to right: Dr. Joanna Porankiewicz-Asplund, Isabel Salén

Take advantage of a new filter function to find the antibody you need

You can now filter the products in any product category, to easier find the item you are looking for.

For all Agrisera primary antibodies, you can filter by:

Confirmed reactivity: The antibody has been confirmed to be reactive in the species in question, experimentally
Predicted reactivity: The antibody has been confirmed to be reactive in the species in question, through sequence homology
Tested applications: The applications in which you can use the antibody in question
Clonality: Polyclonal/monoclonal

For all Agrisera secondary antibodies, you can filter by:

Host: The host species for the antibody in question
Conjugation: ALP, Biotin, a wide range of Fluorencent dyes, HRP and Unconjugated
Tested applications: The applications in which you can use the antibody in question

In case of any questions, you are always welcome to contact us!

Marchantia polymorpha - find suitable antibodies for this model species

Marchantia polymorpha is an interesting model species of large thalloid liverwort, in the class Marchantiopsida. M. polymorpha is highly variable in appearance and contains several subspecies. This species is dioicous, having separate male and female plants. It offers different advantages as a model species, including:

Short life cycle
Ease of propagation and crossing
High frequency of transformation
Haploidy
Small genome (approximately 280 Mb)

Agrisera offers antibodies for various proteins of this model species. Browse available antibodies here.

Can not find an antibody you are looking for? Contact us.

Find out about other alternatives to Arabidopsis thaliana, in the post from Plantae.

Organelle specific HSP70 antibodies

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates, and is highly conserved throughout evolution. Hsp70 works as a molecular chaperone, and plays an important role in how cells cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein, and a member of the family of molecular chaperones.

Below you can fin Agrisera antibodies to plant HPS70 target isoforms, located in different cellular compartments:

These antibodies are developed to short synthetic peptides, unique for each isoform, and each antibody has been used in numerous publications.

Until end of March 2024 all antibodies related to environmental stress are offered with a 20% discount, when using discount code Stress20.

Agrisera Western blot workshop in person at Uppsala University

On February 16th, Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, will conduct a Western blot workshop in person for researchers at Uppsala University. Joanna will share her extensive knowledge and vast experience of 20+ years of using this widely employed protein analysis technique.

The workshop is going to consist of two parts, including a 10-minute break for coffee and the Agrisera Antibody and Western blot Quizzes.

Participants will have a chance to win an Agrisera high-titer secondary antibody and 2 ECL reagents, in quizzes with questions about antibodies and the Western blot technique.

Welcome!

Agrisera Western Blot Workshop at Uppsala University

10% discount on all directly conjugated primary antibodies

Using directly conjugated primary antibodies will help shorten both Western blot and immunolocalization procedures, as the secondary antibody incubation step is not necessary. Agrisera offers directly conjugated, antigen affinity purified primary antibodies to proteins involved in:

Photosynthesis antibodies (PEPC, PRK, PsbA, RbcL, SPS)
Compartment marker antibodies (BiP, Clathrin heeavy-chain, H3, H+ATPase, VDAC1)
Epitope tag antibodies (3xHisBC2, C-YFP, LUC, GFP, GST, N-YFP, TurboID)
Loading control antibodies (H3, RbcL)

These antibodies are offered directly conjugated to:

Alkaline phosphatase (ALP)
Biotin
Fluorophores: DyLight® in the range of 350 to 850 nm
Horse radish peroxidase (HRP)

A primary antibody must be in antigen affinity purified, to allow the conjugation to an enzyme or a dye.
Take a look at a complete list of available directly conjugated primary antibodies here.

The 10% discount for this product group is offered until end of December 2024.

Agrisera directly conjugated primary antibodies for plant and algal research

New free-of-charge images for download added!

As a gift for the plant science community, Agrisera offers over 60 free-of-charge schemes and images for download, to use on your website and in your publications!

New images added, include common plant model species, electron transfer cofactors and AGO proteins.

Please cite SciGrafik and Agrisera as the source for these images.

Browse all the images here.

Free schemes for plant science to download

Agrisera distributor in India, Biogenuix on the Annual Meetiong of Plant Tisssue Culture Association

Between January 23rd and January 25th, one of Agrisera's distributors in India, Biogenuix, attended the 45th Annual Meeting of Plant Tissue Culture Association.

The goal of this meeting was to provide a platform for students, researchers and scientists to present their work, and exchange the most recent developments in plant science.

Agrisera's product catalog and flyers were available for participants.

Biogenuix representing Agrisera at Annual Meeting of Plant Tissue Culture Association

Image courtesy of Biogenuix.

Agrisera Western blot workshop with BiotechnoLabs, Agrisera distributor in India

On January 30th, Agrisera conducted a free online Western blot workshop together with our distributor in India, BiotechnoLabs. The workshop was also opened for worldwide participation. We had the pleasure to connect with researchers from India, Czech Republic, Poland, Spain, Portugal, France, Germany and the UK.

The workshop was presented by Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager. Joanna shared her extensive knowledge and vast experience of 20+ years of using this widely employed protein analysis technique.

The workshop consisted of two parts, including a 10 minute break for coffee and the Agrisera Antibody and Western blot Quizzes, which were very well attended. Many interesting questions were asked.

Participants also had a chance to win an Agrisera high-titer secondary antibody and 2 ECL reagents, in quizzes with questions about antibodies and the Western blot technique.

Each participant of the workshop received a pdf of the presentation, with links to additional information, as well as the recording of the workshop for the future use.

If you are interested in this type of online workshop to be held at your institution, please contact us!

Some feedback we received after the workshop:

"Many thanks for the wonderful presentation. We really enjoyed the informative presentation."

"Thank you again for the very Interesting and Informative Workshop. I would like to ask if It would be possible for me to receive the PowerPoint, since it was very well-made, and I'd like to share it with some coworkers."

"Thank you for such an informative and helpful presentation! You've answered many questions I had (and even some I didn't know I had!) about Western Blots."

"I participated in your amazing presentation today and I would like to receive the recording, presentation and certificate."

Agrisera online Western blot workshop

Images of workshop participants, courtesy of Brajendra Tripathi.

Planning your conference attendance in 2024? Check the Global Plant Events Calendar, for conferences, workshops and online events

The Global Plant Events Calendar, a joint initiative of American Society of Plant Biology (ASPB/Plantae) and Agriera, is providing a quick overview of conferences, congresses, smaller meetings, and online workshops and webinars, with the focus on plant science.

Each year, over 200 events are listed, and the number is continuously growing. The listed events are curated to provide you high-quality events, and protect from irrelevant and so called "predatory" meetings. If you are organizing a meeting, you are welcome to submit it to the calendar. If you are planning your conference attendance, or search for possibilities to increase your knowledge, check the calendar!

For information on how the calendar was created, read an interview conducted by ASPB, with Agrisera's Technical Support Manager, Dr. Joanna Porankiewicz-Asplund, here.

Global Plant Events Calendar, supported by Agrisera

Antibodies to Heat Shock Proteins - useful tools in investigastion of plant responses to environmental stress

Heat Shock Proteins (HSP), are a family of proteins that play essential roles in protecting cells from stressors, such as high temperatures, and are involved in various cellular processes. Agrisera offers specific antibodies to HSP proteins, which can aid research in different areas, such as:

Monitoring Stress Conditions: Antibodies to plant HSPs can be used to monitor the expression levels of these proteins under different stress conditions, such as heat stress, and lead to understanding of the plant's response to environmental challenges.
Understanding Cellular Responses: Antibodies can be employed to determine the cellular localization of specific HSPs. This information is valuable in understanding how these proteins function within different cellular compartments.
Molecular Biology and Biochemistry: Researchers can use antibodies to detect and quantify specific plant HSPs, providing insights into their expression patterns and regulation.
Immunoprecipitation: Antibodies can be used for immunoprecipitation studies to isolate and analyze specific HSP-protein interactions. This helps in understanding the molecular mechanisms underlying stress responses.
Biotechnological applications: Understanding the role of HSPs and their regulation can aid in the development of genetically engineered plants with improved stress tolerance. Antibodies can assist in validating the expression of these proteins in genetically modified plants.
Biomarker development: HSPs may serve as biomarkers for stress in plants. Antibodies can be used to develop diagnostic tools for assessing the stress levels in plants, which is essential for crop management.
Environmental monitoring: Antibodies can be applied to monitor the levels of plant HSPs in natural environments. This can be useful for assessing the impact of environmental stress on plant populations.

The antibodies to plant HSPs are versatile tools that aid researchers in exploring the intricate details of plant stress responses, molecular interactions, and potential applications in agriculture and biotechnology. These antibodies enable scientists to investigate the roles of specific HSPs, assess stress conditions, and develop strategies for improving plant resilience.

Browse the Agrisera HSP antibody collection, offered 20% off until end of March, here.

Agrisera antibodies to HSP proteins, 20 % off until end of March

Agrisera supports the 15th UCMR Day at Ume� University

The 15th UCMR Day took place at Aula Nordica of Umeå University on the 18th of January, 2024.

This one-day conference is aimed at scientists and staff members within Umeå Centre for Microbial Research, UCMR, as well as collaboration partners and other researchers with an interest in microbial research or infection biology. The program offered inspiring keynote lectures, short talks, elevator pitches, and poster presentations, but first and foremost it is an excellent opportunity for networking and initiation of multidisciplinary collaborations.

The winner of Agrisera Best Talk Prize was awarded to PhD student Elin Hahlin, from the Department of Clinical Microbiology at Umeå University, for the talk "Co-infection of HAdV-F41 and AAV-2 as a possible aetiology behind acute hepatitis of unknown origin in children". She will be awarded an antibody of her choice from the Agrisera catalog.

Congratulations and awaiting your free antibody choice!

Phd student Elin Hahlinfrom receives the diploma for Agrisera Best Talk Prize. Picture by Anna Shevtsova.

20% off antibodies to proteins involved in plant environmental stress response

As environmental conditions are changing, plants have to adapt to cope, as they, unlike animals, are stationary and cannot move to another location. Adaptive mechanisms help plants survive and thrive in challenging conditions and include:

Osmotic adjustment
Antioxidant defense system
Change in root morphology
Stomatal regulation
Metabolic adjustment
Dormancy and shedding
Hormonal regulation
Symbiotic relationships
Epigenetic changes

and the synthesis of stress proteins, which help protect cellular structures and enzymes from damage caused by environmental stress.

These mechanisms collectively allow plants to adapt to their environment, and optimize their chances of survival in the face of various stressors. The specific strategies employed can vary depending on both the type of stress and the plant species involved.

Agrisera's antibody collection to proteins involved in plant stress response has been developed over many years, in close collaboration with the scientific community.

You can browse our full environmental stress antibody collection, which is discounted by 20% until end of March 2024. Use the discount code Stress20.

Antibodies to proteins involved in plant heat and drought response

The Global Plant Events Calendar is celebrating 5th anniversary

Exactly 5 years ago, in January 2019, Agrisera and Plantae decided to launch The Global Plant Events Calendar to support the plant science community with a good overview of worldwide meetings, conferences and workshops, both in person and online.

The calendar served as an important resource during the pandemic, when many meetings were cancelled or converted to online events. All events submitted to the calendar are curated, to exclude meetings that do not fit the plant community, as well as so-called "predatory conferences".

"The calendar is about inclusion, education and quality. We promote smaller, local meetings along with the big, popular ones," says Agrisera's Technical Support Manager, Dr. Joanna Porankiewicz-Asplund, in a recent interview conducted by ASPB.

She continues; "And you can think about it like this: if you dive into the cell and look at the organelles and research about it, and then if you take that up and translate it into the tree standing in the forest and being exposed to the climate, the calendar will cover almost every event remotely related to that process. So, this is a metaphor for what this calendar is really covering. It’s all the factors of plant science, internal and external. Big and small."

You are welcome to read the full story of the calendar in the interview by Rachel Belsky, ASPB Peer Review & Content Review Coordinator.

On average, there are around 200 meetings listed in the calendar each year. Over the last few years, the calendar has become recognized by organizers of highly recognized meetings, such as GRC (Gordon Research Conferences) and the Global Plant Council.

5th Anniversary of The Global Plant Events Calendar

Discover the advantages of Agrisera detection reagents for ELISA

Agrisera offers detection reagents for ELISA (Enzyme-Linked Immunosorbent Assay), a widely used laboratory technique, employed for detecting and quantifying the presence of specific proteins, antibodies, peptides, hormones, or other substances in a sample. It is a highly sensitive and specific immunological assay, based on the principles of antigen-antibody interactions.

Visualization of antibody binding is usually performed using a secondary antibody, linked to an enzyme like Alkaline Phosphatase (ALP), or Horse Radish Peroxidase (HRP). A substrate specific to the linked enzyme is then added, and visualization is achieved as described below.

Colorimetric detection: If a chromogenic substrate is used, the enzyme catalyzes a reaction that produces a colored product. The intensity of the color is proportional to the amount of the target substance in the sample. The color change is quantified using a spectrophotometer.

Chemiluminescent detection: If a chemiluminescent substrate is used, the enzyme catalyzes a reaction that produces light. The emitted light is measured using a luminometer. The advantages of chemiluminescent ELISA include its high sensitivity, wide dynamic range, and suitability for quantitative analysis. It is commonly used in various fields, including clinical diagnostics, research laboratories, and pharmaceutical development, where the accurate measurement of protein concentrations is crucial due to its versatility, sensitivity, and ability to handle numerous samples simultaneously.

Until the end of March 2024, Agrisera offers 15% discount on all detection reagents.

Use a discount code: Detect15 and order today!

Agrisera chromogenic and chemiluminescent detection reagents for ELISA offer unique sensitivity at competitive pricing, and skilled technical support to help you to set up the experiments.

Investigation of protein-protein interactions with Agrisera anti-TurboID antibodies

Protein-protein interactions in crop plants can be studied using transient TurboID-based proximity labeling.

In the recent publication "Protocol to identify protein-protein interaction networks in Solanum tuberosum using transient TurboID-based proximity labeling" from the Laboratory of Plant Breeding, Wageningen University & Research, The Netherlands, authors Li Shi et al. described the methodology of transient expression of constructs of a protein under investigation, fused to TurboID, and the identification of protein-protein interactions in potato leaves.

The publication describes a detailed protocol for preparation of plant material and transgene desing, as well as protein identification, using Agrisera's anti-TurboID antibody (AS20 4440). The authors also discuss limitations of the protocol, and provide suggestions on how to adress them.

Steps of the protocol include:

Design and Construction of TurboID Fusion Protein with the protein of interest (POI).
The TurboID enzyme biotinylates nearby proteins within a certain radius (typically tens of nanometers) when activated by the addition of biotin-tyramide and hydrogen peroxide.
Expression of POI with TurboID. After expressing the TurboID fusion proteins, cells are treated with biotin-tyramide and hydrogen peroxide to activate TurboID. TurboID then catalyzes the covalent attachment of biotin to nearby proteins within its labeling radius.
Protein Extraction: Biotinylated proteins are enriched using streptavidin beads or other methods, isolating the proteins that were in close proximity to the TurboID-fused protein.
Protein Identification by Mass Spectrometry. The enriched protein sample is subjected to to mass spectrometry for identification. This helps identify the proteins that were labeled by TurboID.
Validation. Identified protein-protein interactions are often validated using additional experimental techniques, such as Western blot.

Agrisera contributions in 2023 for worldwide plant and algal research community

Agrisera Contribution to Plant and Algal Research community in 2023

In 2023, Agrisera supported the Plant and Algal Research Community worldwide in different ways:

22 Agrisera Best Poster Prizes and Agrisera Best Talk Prizes were awarded at conferences worldwide
Agrisera was present at 7 meetings in 2023, and in total we supported 29 worldwide meetings
Several winners of the Agrisera Online Quizes received either a book on photosynthesis or Agrisera set of high-titer secondary antibodies and ECL reagents
Hundreds of new antibodies were released in 2023
11 technical online workshops were conducted, which gathered hundreds of participants
Technical tips were shared in 14 technical blog posts
Over 2000 hard copies of Agrisera's Educational Posters were distributed to research laboratories worldwide
Over 100 free antibodies were offered to the community

We are happy to contribute to the growth of the scientific community worldwide, and hope to meet you during coming conferences and workshops in 2024!

Happy holidays from Agrisera

Agrisera supports CSPB-SCBV Eastern Regional Meeting 2023

Agrisera supported the Canadian Society of Plant Biologists (CSPB), Eastern Regional Meeting, held the 1st to 2nd of December, at Concordia University, Montreal, Canada.

Meeting topics:

Growth & development
Abiotic stress
Plant-pathogen interactions
Cell biology

Featured Speakers:

Dr. Mehran Dastmalchi, McGill University
Dr. Thomas DeFalco, University of Western Ontario
Dr. Shelley Hepworth, Carleton University

Agrisera's Educational Photosynthesis Posters were available for free for all conference participants, along with the Agrisera Product Catalog, and very popular Western Blot Troubleshooting Guide.

Agrisera at CSPB-SCBV Eastern Regional Meeting 2023

Shipment days at the end of the year 2023

Over the coming holidays, the Agrisera office will be open on all weekdays, except the 25th-26th of December, 2023, and the 1st of January 2024.

The last day for shipments in 2023 will be on Wednesday, December 20th.
Shipments will resume on Monday, January 8th 2024.

Happy Holidays!

Agrisera international, online, Western blot workshop

On the 7th of December, Agrisera conducted an online Western blot workshop together with our distributor in the UK and Ireland, Newmarket Scientific. The workshop was originally dedicated to researchers from the UK and Ireland, but was later opened for worldwide participation. Researchers from 13 countries all around the world joined Agrisera online event. We had the pleasure to connect with researchers from Australia, Japan, India, Czech Republic, Poland, Spain, Portugal, France, UK, Germany, Croatia, Canada and the US.

The workshop was presented by Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager. Joanna shared her extensive knowledge and vast exprience of 20+ years of using this widely employed protein analysis technique.

The workshop consisted of two parts, including a 10 minute break for coffee and the Agrisera Antibody and Western blot Quizzes, which were very well attended. Many interesting questions were asked.

Participants also had a chance to win an Agrisera high-titer secondary antibody and 2 ECL reagents, in quizzes with questions about antibodies and the Western blot technique.

Each participant of the workshop received a pdf of the presentation with links to additional information, as well as the recording of the workshop for the future use.

Some feedback which we received after the workshop:

"The workshop was fascinating; I did not know many details about Western. It was fun!"

"Thank you for all the insights and your expertise ! I will try to convince everyone using the Western blot technique in my lab to optimize their protocol instead of using always the same one !"

"Thank you very much for all the helpful information. "

"The ppt. was very helpful and informative. "

If you are interested in this type of online workshop to be held at your institution, please contact us!

Check new Agrisera 2024 Catalog

The Agrisera 2024 Product Catalog with the most recent product updates and links to product pages, is now available to browse online. The catalog lists following products:

Primary antibodies for research on Plant & Algal Cell Biology, including: loading controls, compartment markers, and specific antibodies to various research areas, such as cell wall, developmental biology, epigenetics, hormones, photosynthesis, environmental stress and more.
Epitope tag antibodies for the most popular, as well as not so commonly used, tags (TurboID, GFP, HIS, FLAG and many more)
High-titer matching secondary antibodies with various labels, such as ALP, HRP, Biotin and DyLights® with the whole wave light spectrum 350-850 nm
Development Reagents for Western blot and ELISA: chromogenic and chemiluminescent of high and medium sensitivity

The catalog also contains useful information about:

The Global Plant Events Calendar, which is a community resource, developed by ASPB/Plantae and Agrisera, that lists both in-person and online conferences, workshops and webinars, all around the world.
Agrisera Educational Posters, developed in collaboration with researchers from specific fields, available for download and to request as hard copies.
Agrisera Free Images, extracted from educational posters, offered for free use in presentations and publications.

You are welcome to browse the online catalog to receive a good overview on Agrisera collection!

To order a card copy of Agrisera 2024 catalog, please contact us.

Agrisera Prize at Ume� Plant Science Centre 2023

Our warmest congratulations to Dr. Marta Derba-Maceluch who received the 2023 Umeå Plant Science Centre (UPSC) Agrisera Prize!

Marta was awarded the prize to acknowledge her significant contributions in cell wall biochemistry research, her dedication to provide excellent technical support for users of the UPSC Microscopy Facility, and her commitment to maintain a safe work environment at UPSC.

The UPSC Agrisera Prize is awarded every year to a person working at UPSC, and this year's prize was announced on the 6th of December during the traditional UPSC Christmas Celebration.

Every year, anyone working at UPSC can nominate a colleague for the award. Based on the written nominations, the members of the UPSC board vote to select the winner, and the prize is a travel voucher, sponsored by Agrisera.

"The prize gives us the opportunity to thank our personnel for their work and commitment. It is always very difficult to choose between the candidates, as they all highly deserve this prize," says Rishikesh Bhalerao who announced the winner of the prize this year.

In the image, left to right: Dr. Catherine Bellini (UPSC), Dr. Marta Derba-Maceluch (UPSC) and Dr. Joanna Porankiewicz-Asplund (Agrisera).

Agrisera in person & online workshop at KBC, Ume� University

On the 29th of November, Agrisera conducted an in-person and online Western blot workshop for researchers from Umeå University, Sweden. In spite of a severe snow storm, many researchers arrived or joined online. The workshop was held by Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager. Joanna shared her extensive knowledge and vast exprience of 20+ years of using this widely employed protein analysis technique.

Participants also had a chance to win an Agrisera high-titer secondary antibody and 2 ECL reagents, in quizzes with questions about antibodies and the Western blot technique.

Each participant of the workshop received a pdf of the presentation with links to additional information, as well as the recording of the workshop for the future use.

Here are some comments we received on the workshop:
"Thank you again for the workshop, I really enjoyed it, and it was informative!"
"Thank you for your time and efforts to put this together!"
"I enjoyed the workshop today. Super informative!"
"Thanks a lot for today's workshop. It was really helpful for a beginner like me."

A warm thank you to the KBC Information Office that helped arrange this event!

Agrisera in person and online workshop at KBC

If you are interested in this type of online workshop to be held at your institution, please contact us!

Agrisera Western blot workshop with Labclinics, Agrisera distributor in Spain & Portugal

On the 28th of November, Agrisera conducted an online Western blot workshop together with our distributor in Spain and Portugal, Labclinics. The workshop was presented by Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager. Joanna shared her extensive knowledge and vast exprience of 20+ years of using this widely employed protein analysis technique.

The workshop consisted of two parts, including a 10 minute break for the Agrisera Antibody and Western blot Quizzes and coffee. Many pitfalls of the Western blot technique were discussed, based on specific experimental examples.

Participants also had a chance to win an Agrisera high-titer secondary antibody and 2 ECL reagents, in quizzes with questions about antibodies and the Western blot technique.

Each participant of the workshop received a pdf of the presentation with links to additional information, as well as the recording of the workshop for the future use. The recording is also available on Agrisera YouTube channel.

If you are interested in this type of online workshop to be held at your institution, please contact us!

Agrisera participates and supports conferences in 2024

Agrisera will participate in the following meetings in 2024

Auxentric 2024, February 27th-29th, Umeå, Sweden
2nd American Photosynthesis Congress, June 3rd-6th, Georgia State University, USA
ASPB's Plant Biology 2024, June 22nd-26th, Honolulu, USA
ICAR 2024, July 15th-19th, UC SanDiego, USA
Plant Meiosis Meeting, September 4th-6th, Poznań, Poland
International Conference of the German Society of Plant Sciences, September 15th-19th, Halle, Germany

Agrisera supports the following meetings in 2024

33rd Western Photosynthesis Conference, January 5th-7th, Oracle, Arizona
15th UCMR Day 2024, January 18th, Umeå University, Sweden
37th Conference Molecular Biology of Plants, March 4th-7th, Germany
41st Eastern Regional Photosynthesis Conference, April 12th-14th, Woods Hole MA, USA
BioLock, Wrocław University, Poland, May
9th Plant Biochemistry Symposium: Plant Metabolites and Signaling, May 13th-14th, Leibniz-Institute of Plant Biochemistry, Halle, Germany
13th International Coference for Plant Mitochondrial Biology, May 26th-30th, St Malo, France
International Society of Root Research, 12th International Symposium, June 2nd-7th, Leipzig, Germany
2nd European Congress on Photosynthesis Research, June, 25th-28th, Padova, Italy
Young Researchers Symposium on Plant Photobiology, July 3rd-5th, Utrecht, The Netherlands
Plant Epigenetics, July 10th-12th, Clermont-Ferrand, France
12th European Workshop on the Biology of Cyanobacteria, September 3rd-6th, Seville, Spain
International Symposium oo Photosynthesis Research in honour of Eva-Mari Aro, September 8th-11th, Turku, Finland
Plant peptides and receptors meeting, September 11th-13th, Wageningen, The Netherlands
12th Plant peptides and receptors meeting, September 11th-13th, Wageningen, The Netherlands
KBC Days 2024, Umeå University, Sweden

In The Global Plant Science Events Calendar, you will find the most up-to-date information about worldwide conferences, congressed and even online events.

This community resource celebrates its 5th anniversary in January 2024.

Agrisera at KBC Days 2023


Between the 7th and 8th of November, Agrisera attended The KBC Days at the Chemical Biological Center of Umeå University The KBC Days is an annual interdisciplinary conference, and this year's theme was "AI in research". The meeting had over 300 participants. PhD students held a 2-minute long elevator picth to present their work. After evaluation of the comeetee, PhD candidate Ololade Awoyomi was awarded The Agrisera Best Elevator Pitch Prize, for the talk "Salvaging dNTPs for mtDNA rescue". Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager participated in the discussion panel "From Academia to Industry", organized by the Postdoc Society. Agrisera will be holding the technical workshop "Mastering Western Blotting Optimization: Expert Tips and Techniques for better results" for KBC in the end of November, in person as well as online. If you are attending this workshop, you are welcome to send in your Western blot questions in advance!		Left to right: Dr. Joanna Porankiewicz-Asplund, at the Agrisera display. Dr. Stefan Björklund, awardee of the best elevator talk, PhD candidate Ololade Awoyomi, Dr. Joanna Porankiewicz-Asplund.

Loading control antibodies to histone 3, are suitable for immunofluorescence

Agrisera loading control antibodies to Histone H3 (AS10 710A) perform well in immunofluorescence, as shown in the image to the right.

Confirmed reactivity of this antibody:
Arabidopsis thaliana, Oryza sativa

Predicted reactivity of this antibody:

Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Physcomitrium patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays Brachypodium distachyon, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Nannochloropsis gaditana, Triticum aestivum, Pinus pinaster, Pisum sativum, Zea mays, Vitis vinifera, Volvox sp.

Species of your interest not listed? Contact us!

Benefit from our Loading Control Antibodies promotion, offering a 20% discount until the end of 2023. Use discount code LC20.

Immunofluorescence using anti-H3 antibodies

The experimental details of the protocol used can be found here, under the Application example tab.

Agrisera antibodies are ranking 31st in the world based on publication record

We are proud to announce that according to a recent publication record analysis, conducted by CiteAB, Agrisera ranks 31st out of the 250 catalog antibodies suppliers that made it on the list. This list includes antibody providers worldwide, and is not limited to plant science.

Plant antibodies have been offered to the community by Agrisera for over 20 years, which has contributed to these excellent results.

Our product information sheets are regularly updated with newly published references.

An overview on Agrisera publication record is available here.

Agrisera supports the 49th Midwest Southeast Photosynthesis Conference

Agrisera supported the 49th Midwest Southeast Photosynthesis Conference, held between the 27th and 29th of October in Turkey Run Inn, Marshall Indiana, USA.

The Midwest Southeast conference is an important platform for discussing new developments in photosynthesis research, from ultrafast spectroscopy to plant biology.

Keynote speakers, 2023:

Dr. Jennifer Bridwell-Rabb: "Metalloproteins in Chlorophyll Biosynthesis"
Dr. Himadri Pakrasi: "Photosynthesis and Nitrogen Fixation in a Unicellular Cyanobacterium"

Agrisera's Educational Photosynthesis Posters are going to be available for free for all conference participants.

Two researchers were awarded the book "Photosynthesis: solar energy for life", as the first prize in a well-attended Agrisera online quiz.

Agrisera supports Midwest Southeast Photosynthesis Conference 2023

Workshop or webinar on demand about Western blot technique

Western blot is the most commony used qualitative protein analysis technique.

If you wish to obtain deeper insights to this technnique, Agrisera offers an on-demand online workshop, presented by our Technical Support Manager, Dr. Joanna Porankiewicz Asplund, who has over 25 years of experience with Western blotting.

The workshop is suitable for any laboratory working with this protein detection method, on a wide range of different samples, like bacterial or algal cultures, cell cultures, as well as plant or animal tissues.

A Q&A session is included in the workshop, and certificates of participation can be issued, if requested. Each participant will receive a pdf of the workshop presentation slides, and a recording of the wokshop.

Take the opportunity and book a Western blot workshop today!

Free epitope tag antibodies and 15 % off until end of the year

Epitope tag antibodies are the most frequently used antibodies in research. If an antibody specific to the protein under investigation is not available, one can instead use its recombinant version, and use an antibody specific to its fusion partner to achieve detection.

Agrisera offers hundreds of epitope tag antibodies, both polyclonal and monoclonal, discounted by 15% until end of 2023.

We are constatly developing new epitope tag antibodies, and currently we are offering the following antibodies free of charge, in return for results:

Ble tag | Cas9 | CBD-Tag | CBP-tag | E Tag | Glu-Glu Tag | Halo Tag | HSV epitope tag | KT3 epitope tag | mStrawberry | Rho1D4/1D4 Tag | S epitope tag | SNAP/Clip Tag | tdTomato | Trx Tag

Check out the full list of antibodies available for testing here.

Contact

Agrisera epitope tag antibodies 15 % off until end of 2023

Labclinics represented Agrisera at 4th Annual Congress of Young Researchers (ACYR)

Agrisera's distributor in Spain, Labclinics, represented Agrisera during the 4th Annual Congress of Young Researchers (ACYR), held between the 9th and 10th of October, at the Center for Research in Agricultural Genomics (CRAG), in Barcelona.

Topics of the sessions included:

Plant responses to stress
Animal and plant genomics
Plant synthetic biology and metabolic engineering
Plant development and signal transduction

Researchers could collect Agrisera Educational Posters and Western blot guides at the Labclinics booth, and ask questions about our products.

Thank you for your interest!

Take advantage of a 20 % discount for all loading control antibodies

Loading controls are useful tools, utilized to confirm that the same amount of protein is loaded across the gel. A good loading control is a housekeeping protein, expressed at high levels, that is not affected by the applied experimental conditions.

Advantages of Agrisera loading control antibodies are:

Loading controls for plant and algal samples
Available off-the-shelf
Tested in a wide range of species
Cited in thousands of publications
Suitable for various applications
Target different comparments and MW

All loading control antibodies can be found here.

Agrisera loading controls are offered at a 20% discount until the end of 2023.

Use discount code: LC20
Valid October-December 2023

20 % off loading control antibodies until end of 2023

Agrisera supports International Conference on Plant Systems Biology and Biotechnology (ICPSBB)

Between the 25th and 27th of September, Agrisera participated in the 2nd International Conference on Plant systems Biology and Biotechnology (ICPSBB), in Plovdiv, Bulgaria.

The sessions covered the following topics:

Abiotic and Oxidative Stress
Bioinformatics and Computational Modelling
Biotechnology
Evolutionary Biology
Genetics and Genomics
Molecular Biology
Molecular Breeding
Plant Signalling and Development
Plant Systems Biology

Agrisera sponsored the Best Poster Prize, which was awarded to Stacey Kerr from the University College Dublin, Ireland. Her poster title was "Using combinatorial transformation to produce valuable metabolites in plants".

The selection committee was composed of Prof. Robert Verpoorte (University of Leiden, The Netherlands), Prof. Paul Dijkwel (Massey University, New Zealand) and Dr. Pavel Kerchev (Mendel University, Czech Republic).

Agrisera supports ICPSBB conference 2023

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2024-04-14 10% discount in return for feedback
2024-04-12 Agrisera supports Eastern Regional Photosynthesis Conference 2024
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2024-04-03 Versatile ECL reagent set, which can save your day!
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2024-03-23 Agrisera supports Jan Andersson Award of the Australian Society of Plant Scientists
2024-03-22

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