Are monoclonal antibodies better, compare to polyclonal?
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2023-11-30
Technical blog
Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?
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2023-09-01
I have not used this antibody before, what conditions of Western blot do you recommend?
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2023-07-27
Which subclasses of immunoglobulin G are found in rabbit serum?
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2023-07-18
Antibody suddenly does not work any longer, what can be the reason?
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2023-07-11
Can an antibody made to a full-length protein distinguish between 2 isoforms?
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2023-05-10
What is a reason for so called "ghost bands" to appear on blots?
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2023-03-30
One of the ways to remove background on a Western blot
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2023-03-24
How to predict in which technique a given antibody may work?
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2023-03-10
Which capture antibody to use in ELISA?
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2023-03-06
Why should we measure protein concentration?
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2023-03-03
Can results obtained in Western blot be representative for other techniques?
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2023-01-31
Effect of a membrane wash on background signal in Western blot
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2022-12-16
How to check predicted species reactivity of an antibody before purchase is done?
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2022-12-15
Which membrane to use for protein transfer?
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2022-11-30
How to visualize a target protein in a Western blot?
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2022-10-31
TBS or TBS-T, how using respective buffer for primary antibody incubation may change the outcome
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2022-10-03
What are the benefits of antibodies provided in lyophilized format?
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2022-10-03
What conditions to apply for detection of low abundance proteins?
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2022-09-09
Can primary antibodies be re-used?
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2022-09-01
How antibodies are validated - interviews with experts
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2022-08-10
Which secondary, fluorescent dye conjugated antibody to use with ECFP/FITC/TRIC filter set?
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2022-08-01
Advice for beginners and experienced users of Western blot technique
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2022-06-20
How to save time and shorten Western blot protocol?
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2022-05-16
How to transfer proteins of low molecular weight?
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2022-03-21
What are the benefits of using directly conjugated primary antibodies?
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2022-03-16
Antigen, immunogen and epitope, what is the difference?
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2022-03-11
How to get an intense signal in Western blot using a weak antibody?
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2022-02-21
How much of a specific antibody can be found in serum?
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2022-01-19
What does confirmed or predicted reactivity of an antibody mean?
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2021-11-26
What determines primary antibody dilution?
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2021-11-15
Affinity purified antibody, what does it really mean for the end user?
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2021-11-10
Can knowing the antigen sequence help identify background bands in a Western blot?
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2021-10-31
Best practices for Western Blot - interviews with experts
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2021-08-18
Store precious samples on a membrane instead of a freezer!
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2021-06-30
Save precious time in the lab, check antigen conservation before antibody purchase
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2021-05-29
Background signal and a sensitivity of ECL reagent
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2021-04-30
Tubulin alpha and gamma are not suitable as a loading control for metal stress
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2021-03-17
What can protein extraction buffer change in a Western blot?
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2021-03-12
Antibody validation strategies - crucial to secure antibody quality
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2021-02-28
What Western blot conditions to apply for high abundance proteins?
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2021-01-13
Same Western blot protocol for all antibodies?
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2020-12-18
Free antibody sample, is it always the best solution?
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2020-11-01
What to consider before antibody purchase?
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2020-10-12
Protein extraction, a key to success in Western blot
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2020-09-30
No signal on Western blot, what to do next?
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2020-08-31
Is sample dentaturation above 95°C, the golden standard?
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2020-07-27
Polyclonal Antibody in serum or affinity purified, what is a difference and how can it influence my results?
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2020-06-30
What are the most important factors for a successful antibody production?
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2020-06-16
Why is pre-immune serum screening of importance before antibody production starts?
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2020-06-08
Technical blog
Latest
Are monoclonal antibodies better, compare to polyclonal?2023-11-30 Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?
2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-18 Antibody suddenly does not work any longer, what can be the reason?
2023-07-11 Can an antibody made to a full-length protein distinguish between 2 isoforms?
2023-05-10 What is a reason for so called "ghost bands" to appear on blots?
2023-03-30 One of the ways to remove background on a Western blot
2023-03-24 How to predict in which technique a given antibody may work?
2023-03-10 Which capture antibody to use in ELISA?
2023-03-06
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