Technical tips

Save precious time in the lab, check antigen conservation before antibody purchase

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2021-05-29

Background signal and a sensitivity of ECL reagent

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2021-04-30

Tubulin alpha and gamma are not suitable as a loading control for metal stress

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2021-03-17

What can protein extraction buffer change in a Western blot?

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2021-03-12

Antibody validation strategies - crucial to secure antibody quality

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2021-02-28

What Western blot conditions to apply for high abundance proteins? 

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2021-01-13

Same Western blot protocol for all antibodies?

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2020-12-18

Free antibody sample, is it always the best solution?

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2020-11-01

What to consider before antibody purchase?

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2020-10-12

Protein extraction, a key to success in Western blot

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2020-09-30

No signal on Western blot, what to do next?

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2020-08-31

Is sample dentaturation above 95°C, the golden standard?

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2020-07-27

Polyclonal Antibody in serum or affinity purified, what is a difference and how can it influence my results?

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2020-06-30

What are the most important factors for a successful antibody production?

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2020-06-16

Why is pre-immune serum screening of importance before antibody production starts?

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2020-06-08

Do secondary antibodies differ only in price?

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2020-06-02

Is the antibody always to blame?

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2020-05-25

TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?

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2020-05-10

What is a specific antibody concentration?

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2020-05-07

FAQs about Catalog Antibodies

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2020-04-23

FAQs about Antibody Production Process

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2020-04-23

What is the safest method to store antibodies?

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2020-03-17

Is an antibody not specific or Western blot protocol is not optimized?

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2020-01-20

Common misconceptions about antibodies - II

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2019-11-25

Common misconceptions about antibodies - I

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2019-10-21

Why fractionate?

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2019-10-14

How to cite the antibodies used in scientific publications?

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2019-03-22

Save time! Use simultaneous incubation with few primary antibodies at the same time.

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2017-04-24

Which secondary antibodies to use when my protein was immunoprecipiated using an antibody?

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2017-02-16

Rapid Western blotting

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2016-05-13

How to know that a commercial antibody is going to work on a protein from my species?

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2014-02-06

Is a standarized reporting of research antibody use - a way to increase experimental reproducibility?

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2013-09-13

Agrisera protein extraction buffer and antibodies used to validate high throughput protein extraction from tough marine phytoplankton

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2010-12-02