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3-hydroxybutyrate dehydrogenase, Biotin conjugated

AS09 563 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)

3-hydroxybutyrate dehydrogenase, Biotin conjugated in the group Antibodies for Plant/Algal / Carbohydrates at Agrisera AB (Antibodies for research) (AS09 563)

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Product Information

Immunogen

3-hydroxybutyrate dexydrogenase isolated and purified from Rhodobacter sphaeroides
Host Rabbit
Clonality Polyclonal
Purity Purified IgG
Format Lyophilized in phosphate buffer saline
Quantity 1 ml
Reconstitution For reconstitution add 0.5 ml of sterile destilled water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 100-1 : 1000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW

26.9 kDa

Reactivity

Confirmed reactivity Rhodopacter shpaeroides
Predicted reactivity

Sagittula stellata, Silicibacter sp.
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.

Related products

Related products

AS09 562 | anti-3-hydroxybutyrate dexydrogenase

AS09 564 | anti-3-hydroxybutyrate dexydrogenase (affinity purified)

Plant protein extraction buffer

Background

Background

3-hydroxybutyrate dehydrogenase (EC=1.1.1.30) is an oxidoreductase which participates in synthesis and degradation of ketone bodies and butanoate metabolism. Alternative names: NAD+-beta-hydroxybutyrate dehydrogenase, hydroxybutyrate oxidoreductase, beta-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase,  -D-hydroxybutyrate dehydrogenase, and beta-hydroxybutyric dehydrogenase.

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Selected references

to be added when available