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Tag Antibody Set 

ABA | Abscisic acid

AS06 195  |  Clonality: Polyclonal  |  Host: Rabbit

ABA | Abscisic acid  in the group Antibodies for Plant/Algal  / Hormones / Abscisic acid at Agrisera AB (Antibodies for research) (AS06 195)

PRODUCT INFORMATION IN PDF

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Datasheet Product citations Add review

Product Information

Immunogen

BSA-conjugated abscisic acid (ABA)

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS, Phosphate 10 mmol/l ; NaCl 150 mmol/l at pH 7.35
Quantity 100 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water and 50 ĩl of glycerol.
Storage Store lyophilized/reconstituted at -20°C; this aliquote can be freezed and thawed for up to five times and showed stability for at least 2 years. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), immunolocalization (IL)
Recommended dilution 1 : 5000-1 : 10 000 (ELISA), 1 : 100 (IL)

Reactivity

Confirmed reactivity Euphorbia pulcherrima
Predicted reactivity Dicots and monocots
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

immunolocalization of ABA in plant tissue

ABA localization in 5 days old roots of Arabidopsis thaliana. ABA signal is shown in red, PIN2 signal shown in green, DAPI in blue. The material has been fixed with 4 % EDAC for 10 min followed by EDAC and para-formaldehyde. Tissue cleaning has been performed before immunolocalization. Rabbit anti-ABA primary antibody diluted in 1: 400, ALEXA 555 conjugated anti-rabbit secondary antibody, diluted 1: 700 (Invitrogen). Co-staining with DAPI visualized nucleus (blue color).

Courtesy Dr. Taras Pasternak, Freiburg University, Germany

Additional information

Additional information Total IgG was purified by ammonium sulfate precipitation and adsorbed on protein carrier.
For detailed immunolocalization protocol check "more information".

Related products

Related products

AS09 446 | ABA | abscisic acid (C1) (for immunolocalization)

AS09 422 | ABA | abscisic acid (C1) (quantity 1mg)

AS09 447 |anti-ABA | abscisic acid (C1) (quantity 5mg)

collection of antibodies to other plant hormones

Background

Background

Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.

Product citations

Selected references

to be added when available