ACCase subunit beta | Acetyl-coenzyme A, carboxylase (subunit beta)
AS15 2880 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Courtesy of Dr. Dr. Elena Pojidaeva Laboratory of Plant Gene Expression Timiryazev Institute of Plant Physiology, Moscow, Russia
Various amounts of recombinant AccD were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.
Reactant: Arabidopsis thaliana (Thale cress)
Application: Western Blotting
Pudmed ID: 33322466
Figure Number: 7A
Published Date: 2020-12-11
First Author: Andreeva, A. A., Vankova, R., et al.
Impact Factor: NoneOpen Publication
Immunoblot analysis of the photosynthetic proteins on the basis of equal total Ponceau S dye stained blot with proteins from leaves of wild type plants and pap1 mutant grown on MS medium in Petri dishes for four weeks under a 16 h light/8 h dark photoperiod at 23 °C with 100 ?E m?2 s?1. Proteins were visualized by immunoblotting using antibodies specific for RbcL, PsaB, PsbD, AtpB, RpoB, AccD and Lhcb2.4 proteins.
ACCase subunit beta (acetyl-coenzyme A, carboxylase (subunit beta)) is a component of the acetyl coenzyme A carboxylase (ACC) complex. It is catalyzing carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT).
Related products: ACCase subunit beta | Acetyl-coenzyme A, carboxylase (subunit beta)
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