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ACCase subunit beta | Acetyl-coenzyme A, carboxylase (subunit beta)

AS15 2880  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana

ACCase subunit beta | Acetyl-coenzyme A, carboxylase (subunit beta) in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS15 2880)

DATA SHEET IN PDF

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Product Information

Immunogen

Recombinant ACC of Arabidopsis thaliana UniProt: P56765, TAIR:ATCG00500

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

For reconstitution add 100 µl of sterile water.

Lyophilized antibody can be stored at -20 or -80°C.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1 : 2500 (WB)
Expected | apparent MW

55.63 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Aethionema grandiflorum, Barbarea verna, Brassica napus, Capsella bursa pastoris, Carica papaya, Cardamine impatiens, Crucihimalaya wallichii, Draba nemorosa, Lepidium virginicum, Lobularia maritima, Nasturtium officinale, Olimarabidopsis pumila, Pachycladon enysii, Raphanus sativus, Theobroma cacao
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

Western blot using anti-ACCase antibodies

The 20 μg of soluble proteins from 14-d-old seedlings of Arabidopsis thaliana were extracted with buffer containing 50 мM Hepes-KOH, pH 7.5, 330 мM sorbitol, 2 мM EDTA, 1 мM MgCl 2, 5 мM ascorbate, 0.05% BSA were mixed with sample buffer and denatured for 5 min at 70°C. Samples were separated on 10% SDS-PAGE and blotted 1h to nitrocellulose membrane (Amersham Protran) using semi-dry transfer (Bio-Rad) in standard transfer buffer in presence of 20% methanol. Transfer of proteins to the membranes was checked using 1% Ponceau S staining before the blocking step. Blots were blocked with 5 % milk in TBS for 1h at room temperature (RT) and then incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1:50 000 (Agrisera) in for 1h at RT with agitation. The blots were washed as above and developed for 5 min with chemiluminescent detection reagent or, alternatively, using Agrisera ECL SuperBright detection reagen (AS16 ECL-S). Images of the blots were obtained using a C-DiGit Scanner (LI-COR).

Courtesy of Dr. Dr. Elena Pojidaeva Laboratory of Plant Gene Expression Timiryazev Institute of Plant Physiology, Moscow, Russia 







Various amounts of recombinant AccD were separated on 12 % SDS-PAGE  and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 5 % milk in TBS  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 10 seconds.

Additional information

Related products

Related products

AS15 2881 | Anti-Biotin carboxyl carrier subunit of chloroplast ACCase, rabbit antibodies

Secondary antibodies

Background

Background

ACCase subunit beta (acetyl-coenzyme A, carboxylase (subunit beta)) is a component of the acetyl coenzyme A carboxylase (ACC) complex. It is catalyzing carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT).

Product citations

Selected references Yu et al. (2018). Starch Deficiency Enhances Lipid Biosynthesis and Turnover in Leaves. Plant Physiol. 2018 Sep;178(1):118-129. doi: 10.1104/pp.18.00539.

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