ACT | Actin (monoclonal, clone 14H4G8), 10 ĩg

AS21 4615-10   | Clonality:  Recombinant monoclonal |  Host:  Mouse recombinant antibody (animal-free technology) | Reactivity: Arabidopsis thaliana

ACT | Actin (monoclonal, clone 14H4G8), 10 ĩg in the group Antibodies for Plant/Algal  / Compartment Markers / Cytoplasm marker at Agrisera AB (Antibodies for research) (AS21 4615-10)


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Product Information

Immunogen ca. 100 amino acids of recombinant actin conserved more than 80 %  in Arabidopsis thaliana: actin-1 P0CJ46  AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293  AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520
Host Mouse
Clonality Recombinant
Subclass/isotype IgG1
Purity Affinity purified, in PBS pH 7.4



 10 µg

Reconstitution For reconstitution add 10 µl, of sterile or deionized water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Recommended dilution 1 : 1000 1: 5000 (WB)
Expected | apparent MW 41.6 | 45 kDa


Confirmed reactivity Arabidopsis thaliana, Zea mays
Predicted reactivity Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao,Triticum aestivum,  Zea mays, Vicia faba
Species of your interest not listed? Contact us
Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-plant actin, mouse recombinant antibodies

10 µg/well of total protein extracted freshly from Arabidopsis thaliana leaf tissue. All lanes shown are from different Arabidopsis thaliana leaf samples extracted simultaneously. Fresh leaf tissue was ground up directly in 1x Bolt LDS loading buffer (Thermo) and 200mM DTT and denatured at 80°C for 10 min. Samples were separated on 12% SDS-PAGE gel and transferred to nitrocellulose by wet transfer for 1hr at 100V. Blot was blocked with 5 % milk in TBST for 1h/RT with agitation. Blot was incubated with Agrisera Mouse anti-actin monoclonal (AS21 4615) at a dilution of 1:1000, in 5% milk with agitation at 1h RT. The blot was rinsed 3 times for 5 minutes in TBS-T with agitation. Then the membrane was incubated with secondary anti-mouse HRP (Agrisera AS09 627) at 1:10 000 dilution in milk for 1hr at room temp. The blots were washed as above and reaction was visualized using ECL reagent and following manufacture's recommendations. The actin band was visualized after 15 seconds of film exposure.

Additional information

This antibody is compatible with a secondary antibody for fluorescence detection:  goat anti-mouse secondary antibody (IRDye 800CW, Li-cor)

Related products

Related products AS13 2640 | Anti-ACT Actin, rabbit antibody

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AS16 3139 | Actin-1, monoclonal antibody
AS16 3140 | Actin-2, mouse monoclonal antibody
AS16 3141 | Actin-8, mouse monoclonal antibody
AS10 702 | Anti-Actin-11, monclonal mouse antibody

Plant protein extraction buffer

Secondary antibodies


Background Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

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