ACT | Actin (monoclonal)

AS16 4111 | Clonality: Monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana

Product Information

Immunogen ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG

Purity Affinity purified, in PBS pH 7.4

Format

Liquid

Quantity

50 µg

Storage

Store at -20°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW 41.6 | 45 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao,Triticum aestivum, Zea mays, Vicia faba
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Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

western blot using anti-plant actin, monoclonal antibodies

50-200µg of total protein from: Arabidopsis thaliana (4), Camelina sativa (5) Arabidopsis lyrata (6) extracted with exaction buffer (50mM Tris-HCl pH7.5, 150mM NaCl, 10%(v/v) glycerol, 0.5mM EDTA and 0.5% NP40) and denatured with SDS-sample buffer at 950°C for 5 min, proteins were separated on 10 % SDS-PAGE and blotted 1h to PVDF membrane using semi-dry transfer. Blots were blocked with 5 % milk for 0.5h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBST at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-mouse IgG (H&L), horse radish peroxidase conjugated, AS09627) diluted to 1: 5 000 in for 1h/RT with agitation. The blot was washed as above, developed for 5 mins with Agrisera ECL Bright and detected with film.

Courtesy Dr Jiaqi Sun, McGill University, Montreal, Canada

Additional information

Related products

Related products AS13 2640 | Anti-ACT Actin, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Background

Background Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.