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AgriseraELISA SuperBright is specially formulated for HRP microwell and ELISA system detection. It is a high quality substrate for detection of horseradish peroxidase enzyme activity at true extreme low femtogram levels, offering low background and superior signal to noise ratio.
It is a ready to use 2 component system (A+B).
Detection conditions need to be optimized for each assay system. This reagent is extremely sensitive and very high HRP concentration can "burn out" the substtrate. This substrate has a very wide detection range for HRP enzyme in a soluble environment.
HS code for this product is: 3822.00.0002.
It is a ready to use 2 component system (A+B).
Product Information
Storage
18 month shelf life at 2°C to 8°C.Mixed working reagent is stable for several days at room temperature or at 4°C. Exceptional lot to lot consistency. Superior stability.
Tested applications
ELISA (ELISA), Microwell application (MA)
Reactivity
Application examples
Additional information
Additional information
This reagent offers extreme low femto level sensitivity combined with low background and superior signal to noise ratios
User Instruction
- Store reagents A and B in the darkness at 4-8°C.
- Mix equal volumes of reagent A and B (chemiluminescent substrate) in a clean container and equilibrate to room temperature 30 minutes before use.
- Do not contaminate the HRP enzyme substrate with HRP or other proteins. Never pipette directly from the bottle stock or pour aliquoted solution back into the stock bottle.
- 100 µl of Agrisera ELISA-ECL should be used/well.
- Incubate at RT for optimial detection e.g. for example 20 min. after substrate contact.
- Assay in appropriate microtieter plates designed for chemiluminescence.
Detection conditions need to be optimized for each assay system. This reagent is extremely sensitive and very high HRP concentration can "burn out" the substtrate. This substrate has a very wide detection range for HRP enzyme in a soluble environment.
HS code for this product is: 3822.00.0002.
Background
Product citations
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Agrisera AB Box 57, SE-911 21 Vännäs, SWEDEN | Phone: +46(0)935 33000 | 
