AgriseraELISA SuperBright (10 ml trial pack)
- Data sheet
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AgriseraELISA SuperBright is specially formulated for HRP microwell and ELISA system detection. It is a high quality substrate for detection of horseradish peroxidase enzyme activity at true extreme low femtogram levels, offering low background and superior signal to noise ratio.
It is a ready to use 2 component system (A+B). - Product Info
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Quantity: 2 x 5 ml, two component ready to use solutions 2 x 50 ml, two component ready to use solutions 2 x 500 ml, two component ready to use solutions Storage: 18 month shelf life at 2°C to 8°C.Mixed working reagent is stable for several days at room temperature or at 4°C. Exceptional lot to lot consistency. Superior stability. Tested applications: ELISA (ELISA), Microwell application (MA) - Additional Information
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Additional information: This reagent offers extreme low femto level sensitivity combined with low background and superior signal to noise ratios Additional information (application): User Instruction
- Store reagents A and B in the darkness at 4-8°C.
- Mix equal volumes of reagent A and B (chemiluminescent substrate) in a clean container and equilibrate to room temperature 30 minutes before use.
- Do not contaminate the HRP enzyme substrate with HRP or other proteins. Never pipette directly from the bottle stock or pour aliquoted solution back into the stock bottle.
- 100 µl of Agrisera ELISA-ECL should be used/well.
- Incubate at RT for optimial detection e.g. for example 20 min. after substrate contact.
- Assay in appropriate microtieter plates designed for chemiluminescence.
Detection conditions need to be optimized for each assay system. This reagent is extremely sensitive and very high HRP concentration can "burn out" the substtrate. This substrate has a very wide detection range for HRP enzyme in a soluble environment.
HS code for this product is: 3822.00.0002. - Protocols
- Antibody protocols
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Accessories
AS15 Diluent | TMB based, especially formulated with extreme sensitivity, HRP substRate for microwell application.
AS18 MacroStab | Agrisera Universal Stabilizer preserves and stabilizes the activity of HRP or ALP conjugates and proteins and other bio-molecules. It is designed as a ready to use stabilizer, buffer, diluent and possible coat block for immunoassays.
This product can be purchased in 3 different volumes:
AS18 Macrostab-10, 10 ml. Trial size limited to one per customer
AS18 Macrostab-100, 100 ml
AS18 Macrostab-1L, 1L
Choose the appropriate volume in the drop down menu to the right
AS15 HRPstab | Bovine and mammalian protein free HRP and ALP-conjugate and protein stabilizer
This product can be purchased in 2 different volumes:
AS15 HRPstab-10, 10 ml. Trial size limited to one per customer
AS15 HRPstab-100, 100 ml
Choose the appropriate volume in the drop down menu to the right
- 10ml trial size is restricted to one unit per customer -
This product is designed to stabilize coated ELISA plates and other solid phase substrates.