AgriseraELISA SuperBright

• 18 month shelf life at 2°C to 8°C.
• Mixed working reagent is stable for several days at room temperature or at 4°C.
• Exceptional lot to lot consistency.
• Superior stability.

User Instruction

• Store reagents A and B in the darkness at 4-8°C.
• Mix equal volumes of reagent A and B (chemiluminescent substrate) in a clean container and equilibrate to room temperature 30 minutes before use.
• Do not contaminate the HRP enzyme substrate with HRP or other proteins. Never pipette directly from the bottle stock or pour aliquoted solution back into the stock bottle.
• 100 µl of Agrisera ELISA-ECL should be used/well.
• Incubate at RT for optimial detection e.g. for example 20 min. after substrate contact. 
• Assay in appropriate microtieter plates designed for chemiluminescence.