NRT1.4 | Nitrate transporter
AS09 473 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Raphanus sativus
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63.5 | 70 kDa (Arabidopsis thaliana)
Arabidopsis thaliana, Raphanus sativus
5 µg and 10 µg of vacuolar membrane fraction/lane from Raphanus sativus were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-AJ011604 | nitrate transporter antibodies (AS09 473, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.
This protein is present in plant tissues in very low levels.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Nitrate transporter functions in transporter activity in response to jasmonic acid stimulus and response to wounding. This protein is coded by ntp2 gene.
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