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A1i3 | alpha-1-inhibitor 3

AS07 272  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Rat

A1i3 | alpha-1-inhibitor 3 in the group Antibodies Human Cell Biology / Other proteins at Agrisera AB (Antibodies for research) (AS07 272)



DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

Purified Rat alpha-1-inhibitor 3 P14046
Host Rabbit
Clonality Polyclonal
Purity Total IgG. Protein G purified in PBS pH 7.4.
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

164 | 180 kDa

Reactivity

Confirmed reactivity Rat
Predicted reactivity Rat
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

application example - Western blot

 

Experimental conditions:

Blotting with rabbit anti-rat alpha-1-inhibitor 3. Ten ml of rat plasma, diluted 200 times with PBS, and 20 ng purified rat alpha-1-inhibitor 3 were separated on a 9% polyacrylamide gel, transferred to a PVDF membrane, blocked, incubated 2 h at RT with anti-alpha-1-inhibitor 3 IgG, 1 mg/ml, washed and then incubated for 30 min with swine anti-rabbit IgG-HRP and washed. The membrane was developed using the ECL system.
 
 
https://www.agrisera.com/dokument/bibliotek/Image/AS07%20272%20western%20blot.jpg

 

 

Additional information

Related products

Background

Background

Rat alpha-1-inhibitor 3 (A1i3) is a proteinase inhibitor in rat plasma. It belongs to the same superfamily as human alpha-2-macroglobulin (a2M). Alternative names: Alpha-1-inhibitor 3 variant II antibody, Alpha-1-inhibitor III antibody

Product citations

Selected references Kim et al. (2008) 2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter. Proteomics, 8(17):3632-44.
immunogen:

Purified Rat alpha-1-inhibitor 3 P14046
Reconstitution: For reconstitution add 200 ĩl of sterile water
Host: Rabbit
Clonality: Polyclonal
Purity: Total IgG. Protein G purified in PBS pH 7.4.
Format: Lyophilized
Quantity: 200 ĩg
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution: 1 : 1000 (WB)
calculated | apparent molecular mass [kDa]:

164 | 180 kDa
Confirmed reactivity: Rat
predicted reactivity: Rat
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer):

application example - Western blot

 

Experimental conditions:

Blotting with rabbit anti-rat alpha-1-inhibitor 3. Ten ml of rat plasma, diluted 200 times with PBS, and 20 ng purified rat alpha-1-inhibitor 3 were separated on a 9% polyacrylamide gel, transferred to a PVDF membrane, blocked, incubated 2 h at RT with anti-alpha-1-inhibitor 3 IgG, 1 mg/ml, washed and then incubated for 30 min with swine anti-rabbit IgG-HRP and washed. The membrane was developed using the ECL system.
 
 
https://www.agrisera.com/dokument/bibliotek/Image/AS07%20272%20western%20blot.jpg

 

 
background:

Rat alpha-1-inhibitor 3 (A1i3) is a proteinase inhibitor in rat plasma. It belongs to the same superfamily as human alpha-2-macroglobulin (a2M). Alternative names: Alpha-1-inhibitor 3 variant II antibody, Alpha-1-inhibitor III antibody
All references: Kim et al. (2008) 2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter. Proteomics, 8(17):3632-44.

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