Name:
Phone:
E-mail:
Message

Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

A1i3 | alpha-1-inhibitor 3

AS07 272  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Rat

A1i3 | alpha-1-inhibitor 3  in the group Human/Animal Antibodies / Human Proteins / Other Human proteins at Agrisera AB (Antibodies for research) (AS07 272)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Info: Add review
product information
Background

Rat alpha-1-inhibitor 3 (A1i3) is a proteinase inhibitor in rat plasma. It belongs to the same superfamily as human alpha-2-macroglobulin (a2M). Alternative names: Alpha-1-inhibitor 3 variant II antibody, Alpha-1-inhibitor III antibody

Immunogen

Purified Rat alpha-1-inhibitor 3 P14046

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products
Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

164 | 180 kDa

Confirmed reactivity Rat
Predicted reactivity Rat
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Kim et al. (2008) 2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter. Proteomics, 8(17):3632-44.

application example - Western blot

 

Experimental conditions:

Blotting with rabbit anti-rat alpha-1-inhibitor 3. Ten ml of rat plasma, diluted 200 times with PBS, and 20 ng purified rat alpha-1-inhibitor 3 were separated on a 9% polyacrylamide gel, transferred to a PVDF membrane, blocked, incubated 2 h at RT with anti-alpha-1-inhibitor 3 IgG, 1 mg/ml, washed and then incubated for 30 min with swine anti-rabbit IgG-HRP and washed. The membrane was developed using the ECL system.
 
 
https://www.agrisera.com/dokument/bibliotek/Image/AS07%20272%20western%20blot.jpg

 

 


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com