A1i3 | alpha-1-inhibitor 3

AS07 272  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Rat

A1i3 | alpha-1-inhibitor 3  in the group Antibodies Human Cell Biology Research / Other proteins at Agrisera AB (Antibodies for research) (AS07 272)


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Product name, number (Agrisera, Sweden)

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Product Information


Purified Rat alpha-1-inhibitor 3 P14046

Host Rabbit
Clonality Polyclonal
Purity Total IgG. Protein G purified in PBS pH 7.4.
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

164 | 180 kDa


Confirmed reactivity Rat
Predicted reactivity Rat
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

application example - Western blot


Experimental conditions:

Blotting with rabbit anti-rat alpha-1-inhibitor 3. Ten ml of rat plasma, diluted 200 times with PBS, and 20 ng purified rat alpha-1-inhibitor 3 were separated on a 9% polyacrylamide gel, transferred to a PVDF membrane, blocked, incubated 2 h at RT with anti-alpha-1-inhibitor 3 IgG, 1 mg/ml, washed and then incubated for 30 min with swine anti-rabbit IgG-HRP and washed. The membrane was developed using the ECL system.



Additional information

Related products



Rat alpha-1-inhibitor 3 (A1i3) is a proteinase inhibitor in rat plasma. It belongs to the same superfamily as human alpha-2-macroglobulin (a2M). Alternative names: Alpha-1-inhibitor 3 variant II antibody, Alpha-1-inhibitor III antibody

Product citations

Selected references Kim et al. (2008) 2-D DIGE and MS/MS analysis of protein serum expression in rats housed in concrete and clay cages in winter. Proteomics, 8(17):3632-44.

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