Alkaline lipase (glyoxysomal lipase)

AS10 698 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ricinus communis

Product Information

Immunogen

native, purified alkaline lipase from Ricinus communis

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 2000 (WB)

Expected | apparent MW

62 kDa

Reactivity

Confirmed reactivity Coffea arabica, Ricinus communis

Predicted reactivity Higher plants
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Method for preparation of plant glyoxysomal membrane.

Serum contains less than 0.1 % of sodium azide as preservative.

Related products

antibodies to proteins involved in plant lipid metabolism

Plant protein extraction buffer

Secondary antibodies

Background

Alkaline lipase is a glyoxysomal enzyme which breaks down fat molecules. Endogenous storge lipid in Ricinus communis, tricinolein is hydrolyzed by this enzyme, localized in the glyoxysomes of endosperm.

Product citations

Selected references Patui et al. (2014). Lipase activity and antioxidant capacity in coffee (Coffea arabica L.) seeds during germination. Plant Sci. 2014 Apr;219-220:19-25. doi: 10.1016/j.plantsci.2013.12.014. Epub 2014 Jan 3.