Agrisera Super Deal

Primary/Secondary/ECL

Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)

- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright, for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

AMBP | Alpha-1-microglobulin (clone BN11.10)

AS01 013 | Clonality: monoclonal | Host: Mouse | Reactivity: Human, Rat

PRODUCT INFORMATION IN PDF

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product information
Background		Alpha-1-microglobulin is a 27-kDa glycoprotein with immunosuppressive properties. A gene AMBP in the lipocalin cluster encodes alpha-1-microglobulin together with a Kunitz-type proteinase inhibitor, bikunin. The gene is translated into the alpha-1-microglobulin-bikunin precursor, which is subsequently cleaved and the two proteins are secreted to the blood separately. Alpha-1-microglobulin is found in blood and in connective tissue in most organs. It is most abundant at interfaces between the cells of the body and the environment, such as in lungs, intestine, kidneys and placenta. Alpha 1-microglobulin is an immunomodulatory protein with a broad spectrum of possible clinical applications and seems a promising marker for evaluation of tubular function.
Immunogen		human and rat alpha-1-microglobulin, native, full length, purified from urine, UniProt: P02760 cleavege product no. PRO_0000017886
Host		Mouse
Clonality		Monoclonal
Clone		IgG2a;
Purity
Format		Lyophilized
Quantity		50 µg
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		ImmunoAffinity chromatography (IAC), ELISA (ELISA), Immunohistochemistry (IHC), immunoflourescence (IF), Immunoprecipitation (IP),, Radioimmunoassay (RIA), Western blot (WB)
Related products		AS01 014 \| anti-rat alpha-1 microglobulin Secondary antibodies
Additional information		Antibody is purified and contains 0.5 mg/ml of mouse albumin in 150 mM NaCl, 20 mM Tris-HCl, pH 8 and 0.02% sodium azide;This Antibody is also reacting with the AMBP precursor.

application information
Recommended dilution		0.2-2 mg/L (ELISA), 5 µg/µl (IP), 20 ng/ml (RIA), 1 µg/ml (IHC), 10 mg/1ml of gel (ACH), 5 µg/ml (IF), 1 : 2000 (WB)
Expected \| apparent MW		27 kDa
Confirmed reactivity		Human, guinea pig, Rabbit, Rat
Predicted reactivity
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		For usage on rabbit and guinea pig the antibody has to be applied in high concentrations.The antibody has been used on frozen sections only, not paraffin. Suggested antigen retrieval is 5 min in microwave owen in citrate buffer, pH 6.
Selected references		Olsson et al. (2008). The lipocalin alpha1-microglobulin protects erythroid K562 cells against oxidative damage induced by heme and reactive oxygen species. Free Radic. Res. 42:725-732; Yokota et al. (2007) Absence of increased alpha1-microglobulin in IgA nephropathy proteinuria. Mol Cell Proteomics, 6:738-44.

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com