AMBP | Alpha-1-microglobulin (clone BN11.10)

AS01 013 | Clonality: monoclonal | Host: Mouse | Reactivity: Human, Rat

Product Information

Immunogen

human and rat alpha-1-microglobulin, native, full length, purified from urine, UniProt: P02760 cleavege product no. PRO_0000017886

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG2a

Format Lyophilized

Quantity

50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ImmunoAffinity chromatography (IAC), ELISA (ELISA), Immunohistochemistry (IHC), immunoflourescence (IF), Immunoprecipitation (IP),, Radioimmunoassay (RIA), Western blot (WB)

Recommended dilution 0.2-2 mg/L (ELISA), 5 µg/µl (IP), 20 ng/ml (RIA), 1 µg/ml (IHC), 10 mg/1ml of gel (ACH), 5 µg/ml (IF), 1 : 2000 (WB)

Expected | apparent MW

27 kDa

Reactivity

Confirmed reactivity Human, guinea pig, Rabbit, Rat

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Antibody is purified and contains 0.5 mg/ml of mouse albumin in 150 mM NaCl, 20 mM Tris-HCl, pH 8 and 0.02% sodium azide;This Antibody is also reacting with the AMBP precursor.

For usage on rabbit and guinea pig the antibody has to be applied in high concentrations.The antibody has been used on frozen sections only, not paraffin. Suggested antigen retrieval is 5 min in microwave owen in citrate buffer, pH 6.

Related products

AS01 014 | anti-rat alpha-1 microglobulin

Background

Alpha-1-microglobulin is a 27-kDa glycoprotein with immunosuppressive properties. A gene AMBP in the lipocalin cluster encodes alpha-1-microglobulin together with a Kunitz-type proteinase inhibitor, bikunin. The gene is translated into the alpha-1-microglobulin-bikunin precursor, which is subsequently cleaved and the two proteins are secreted to the blood separately. Alpha-1-microglobulin is found in blood and in connective tissue in most organs. It is most abundant at interfaces between the cells of the body and the environment, such as in lungs, intestine, kidneys and placenta. Alpha 1-microglobulin is an immunomodulatory protein with a broad spectrum of possible clinical applications and seems a promising marker for evaluation of tubular function.

Product citations

Selected references

Olsson et al. (2008). The lipocalin alpha1-microglobulin protects erythroid K562 cells against oxidative damage induced by heme and reactive oxygen species. Free Radic. Res. 42:725-732;
Yokota et al. (2007) Absence of increased alpha1-microglobulin in IgA nephropathy proteinuria. Mol Cell Proteomics, 6:738-44.