Agrisera Super Deal

Primary/Secondary/ECL

Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)

- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright, for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

AMBP | Alpha-1-microglobulin

AS01 014 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Rat

PRODUCT INFORMATION IN PDF

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product information
Background		Alpha-1-microglobulin is a 27-kDa glycoprotein with immunosuppressive properties. A gene AMBP in the lipocalin cluster encodes alpha-1-microglobulin together with a Kunitz-type proteinase inhibitor, bikunin. The gene is translated into the alpha-1-microglobulin-bikunin precursor, which is subsequently cleaved and the two proteins are secreted to the blood separately. Alpha-1-microglobulin is found in blood and in connective tissue in most organs. It is most abundant at interfaces between the cells of the body and the environment, such as in lungs, intestine, kidneys and placenta. Alpha 1-microglobulin is an immunomodulatory protein with a broad spectrum of possible clinical applications and seems a promising marker for evaluation of tubular function.
Immunogen		rat alpha-1-microglobulin, full length, recombinant protein, overexpressed in baculovirus-infected insect cells with N-terminal His-tag
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized with 0.04% sodium azide
Quantity		200 µl
Reconstitution		For reconstitution add 200 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Immunocytochemistry (ICC), Radioimmunoassay (RIA), Western blot (WB)
Related products		AS01 013 \| alpha-1-microglobulin (abcam BN11.10), mouse monoclonal antibody AS13 2670 \| A1M \| human alpha-1-microglobulin, chicken antibody AS10 1354 \| B2M \| beta-2-microglobulin, goat antibody Secondary antibodies
Additional information

application information
Recommended dilution		1 : 5000 (ELISA), 1 : 200 (IHC), 1 : 6000 (RIA), 1 : 2000 (WB)
Expected \| apparent MW		27 kDa
Confirmed reactivity		Rat
Predicted reactivity		Mouse
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		Wen et al. (2017). MicroRNA-148b regulates megalin expression and is associated with receptor downregulation in mice with unilateral ureteral obstruction. Am J Physiol Renal Physiol. 2017 Mar 22:ajprenal.00585.2016. doi: 10.1152/ajprenal.00585.2016. Sanchez et al. (2002) Expression of the AMBP gene transcript and its two protein products, alpha(1)-microglobulin and bikunin, in mouse embryogenesis. Mech Dev, 117:293-8. Ostendorf et al. (2006) Antagonism of PDGF-D by human antibody CR002 prevents renal scarring in experimental glomerulonephritis. J Am Soc Nephrol, 17:1054-62.

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