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GnRHa | gonadotropin-releasing hormone agonist (200 ĩl)

AS05 087  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: GnRHa

GnRHa | gonadotropin-releasing hormone agonist (200 ĩl) in the group Human/Animal Antibodies / Fish Proteins at Agrisera AB (Antibodies for research) (AS05 087)

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Background

Gonadotropin-releasing hormone (GnRH) is considered to stimulate LH secretion from the pituitary. This antibody is raised against an GnRH analog (GnRHa).

Immunogen

GnRHa (des-Gly10, D-Ala6, Pro9-NHEt)-LHRH pGlu-His-Trp-Ser-Tyr-D-Ala-Leu-Arg Pro-NHEt C56H78N16O12, FW 1167

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA)
Related products

AS05 087-20 | anti-GnRHa, smaller unit size of AS05 087

AS05 088 | anti-GnRHa hen antibody

Secondary antibodies

Additional information
application information
Recommended dilution

1: : 80 000 (ELISA)

Expected | apparent MW
Confirmed reactivity GnRHa
Predicted reactivity

GnRHa

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

In ELISA immunoassay anti-GnRHa antibody and sample/standard are added simultaneously within GnRHa coated wells. After incubation, anti-GnRHa antibody bound to the coated GnRHa is detected with HRP-labelled secondary antibodies and color development achieved by addition of TMB reagent.

Selected references Amezawa et al. (2018). Spawning induction of blue mackerel Scomber australasicus and eastern little tuna Euthynnus affinis by oral administration of a crude gonadotropin-releasing hormone analogue. . Fish Sci (2018). https://doi.org/10.1007/s12562-018-1185-6. Guzmán et al. (2008). Vitellogenin, steroid plasma levels and spawning performance of cultured female Senegalese sole (Solea senegalensis. General & Comparative Endocrinol. 156: 285-297.


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